Laboratory apparatus for optional temperature-controlled heating and cooling

• US 5,061,630 A
• Filed: 05/12/1989
• Issued: 10/29/1991
• Est. Priority Date: 05/13/1988
• Status: Expired due to Term

• Current Assignee: K-Midt Ltd. Liab., Co., Ulrich C. Knopf
• Original Assignee: Agrogen Foundation Seyffer & Company & Ulrich C Knopf
• Inventors: Sieber, Joseph, Knopf, Ulrich C.
• Primary Examiner(s): Dority, Carroll B.
• Application Number:
  US07/350,803
• Time in Patent Office: 900 Days
• Field of Search: 62/3.3, 62/3.62, 62/457.9, 435/290, 435/287
• US Class Current: 422/500
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: F25B 21/04 reversible

Abstract

Laboratory apparatus for optionally heating or cooling samples, comprising a block of one or more Peltier elements which, with one of their thermal poles, are in thermal contact with an essentially rectangular block of heat conducting metal, and with the other pole are in thermal contact with a heat exchanger, this latter being thermally insulated from the metal block. One of the outer surfaces of the rectangular metal block serves as a working outer surface for heating or cooling the samples. All outer surfaces of the metal block, with the excption of the working surface and the surface in contact with the Peltier elements, are thermally insulated. The working surface may be used for heating or cooling at will through the inversion of the direction of the alimentation current for the Peltier elements.

Claims

1. Laboratory apparatus for optionally heating or cooling samples, comprising a block of one or more Peltier elements which, with one of their thermal poles, are in thermal contact with an essentially rectangular block of heat conducting metal, and with the other pole are in thermal contact with a heat exchanger, the heat exchanger being thermally insulated from the metal block, one of the outer surfaces of the rectangular metal block being a substantially flat working surface for heating or cooling the samples, and all outer surfaces of the metal block, with the exception of the working surface and the surface in contact with the Peltier elements, being thermally insulated, whereby the working surface may be used for heating or cooling at will through inversion of the direction of the alimentation current for the Peltier elements.

2. Apparatus according to claim 1, characterized in that the heat conducting metal is aluminum or stainless steel.

3. Apparatus according to claim 1 characterized in that the heat exchanger is provided with channels through which a heat transporting liquid may be conducted.

4. Apparatus according to claim 1 characterized in that the heat exchanger is a metallic block provided with ribs, over which an air stream is blown by a ventilator.

5. Apparatus according to claim 1 comprising more than one Peltier element, characterized in that at least two out of the total number of Peltier elements are thermally arranged in series, said Peltier elements being arranged in a vertical stack with the opposite poles of two neighbouring elements in contact with one another.

6. Apparatus according to claim 1 characterized in that the current for the alimentation of the Peltier elements is governed by a control circuit comprising a temperature sensor.

7. Apparatus according to claim 1 characterized in that it comprises an exchangeable working module for holding vessels containing the samples to be heated or cooled, said working module comprising, on the one side, a face for contact with the flat working surface, and on the other side cavities for receiving the sample vessels.

8. Apparatus according to claim 7, characterized in that the working module is a rectangular block whose upper surface is provided with openings in which to receive the sample vessels and whose base serves as a contact surface to the working surface of the metal block, the remaining outer surfaces of the working module being thermally insulated.

9. Apparatus according to claim 7, characterized in that the working module comprises an open trough made of heat conducting metal which is provided with a base plate, which at least partially extends beyond the trough at its sides, the lower surface of this base plate serving for contact with the working surface of the metal block, the trough and the base plate, except its lower surface, being covered by an insulating layer.

10. Apparatus according to claim 9, characterized in that the trough of the working module is filled by a liquid and in that the trough includes a cover.

11. Apparatus according to claim 9, characterized in that the trough of the working module is filled by solid particles whose diameter does not exceed 5 millimeters, whereby the sample vessels may be positioned between the solid particles.

12. Apparatus according to claim 11, characterized in that the solid particles are spheres of metal or glass.

13. Apparatus according to claim 11, characterized in that the interstices between the solid particles are filled by liquid.

14. Apparatus according to claim 9, characterized in that the open trough is equipped with interchangeable inserts, which are provided with openings for receiving the vessels containing the samples.

15. Apparatus according to claim 1, further comprising a power supply for the Peltier elements, an electronic control circuit, a temperature indicating instrument and a commutator for choosing heating or cooling modes contained in a separate housing, this latter being connected by an electric cable to the Peltier elements, whereby a battery or a mains connection are used optionally for the electric alimentation.

16. Apparatus according to claim 15, characterized in that the control circuit is equipped with two temperature sensors, one of them measuring the temperature of the metal block in contact with the Peltier elements, and the other measuring the temperature of the sample, whereby, according to choice, any of the two sensors may be used separately, or both together to influence the control circuit.

17. Apparatus according to claim 15, comprising an alimentation and control unit provided with a microcomputer incorporated in, or externally connected thereto, this microcomputer being programmable for executing temperature cycles, whereby, if necessary, the temperatures in the cycle may be situated partly below and partly above ambient temperature and whereby, during the cycle, the apparatus is switched automatically from cooling to heating mode and vice-versa.

18. Apparatus according to claim 7, wherein the working module to be brought into contact with the metal block is equipped with interior channels for the transport of a liquid, whereby this liquid is either the sample itself or a heat transporting medium.

19. Apparatus according to claim 1, characterized in that a pulsed power supply is used for the alimentation of the Peltier elements.