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Integral multilayer analytical element

  • US 5,063,153 A
  • Filed: 10/09/1987
  • Issued: 11/05/1991
  • Est. Priority Date: 10/09/1986
  • Status: Expired due to Term
First Claim
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1. A process for the preparation of a dry integral multilayer analytical element having reagent layers containing a dehydrogenase, oxidized nicotinamide coenzyme, an electron transport compound, an electron acceptable dye-forming compound, an alkali agent or an alkaline buffer selected from the group consisting of 2-amino-2-methyl-1,3-propanediol, 2-amino-2-ethyl-1,3-propanediol, diethanolamine, ethanolamine, 2-amino-2-methyl-1-propanediol, tris(hydroxymethyl)aminomethane and trimethylamine provided on a light-transmissive, water-impermeable support which comprises the steps of:

  • forming on said support a reagent layer containing at least said oxidized nicotinamide coenzyme and electron acceptable dye-forming compound dispersed in a hydrophilic polymer;

    forming a spreading layer having a metering effect;

    coating a solution or dispersion containing at least said alkali agent or alkaline buffer in an organic solvent which causes substantially no swelling of the hydrophilic polymer in the underlying reagent layer over the spreading layer; and

    drying the coated layers.

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