Kit for analysis of subsets of subpopulations of leukocytes

• US 5,064,616 A
• Filed: 09/17/1990
• Issued: 11/12/1991
• Est. Priority Date: 11/30/1987
• Status: Expired due to Fees

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Brosnan, Jeanne, Ault, Kenneth
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Trautman, Kimberly A.
• Application Number:
  US07/583,532
• Time in Patent Office: 421 Days
• Field of Search: 422/44, 422/61, 422/50, 422/99, 436/56, 436/501, 436/546, 436/805, 436/808, 435/2, 435/4, 435/7.24, 435/808, 356/39, 356/73
• US Class Current: 422/430
• CPC Class Codes: G01N 15/1456 without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
• CPC Class Codes: G01N 33/50 Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing measuring or testing processes involving enzymes or microorganisms, compositions or test papers therefor; processes for forming such compositions, condition responsive control in microbiological or enzymological processes C12Q
• CPC Class Codes: G01N 33/56972 White blood cells

Abstract

A kit for determination of subsets of leukocytes utilizing flow cytometry analysis techniques.

Claims

1. A kit for determination of subsets of a subpopulation of leukocytes in a sample containing a first subpopulation of leukocytes of interest and a second subpopulation of leukocytes which are not of interest, said kit comprising;

a first monoclonal antibody which is labeled with a first fluorochrome and which reacts with substantially all leukocytes, a second monoclonal antibody which is labeled with a second fluorochrome and reacts with at least a subset of leukocytes, a third monoclonal antibody which is labeled with a third fluorochrome and which reacts with a first subset of leukocytes within said first subpopulation of interest and a fourth monoclonal antibody which is labeled with a fourth fluorochrome and which reacts with a second subset of leukocytes within said first subpopulation of interest, said first and second fluorochromes and said third and fourth fluourochromes having distinct and different emission spectra.

2. A kit in accordance with claim 1 which also includes an IgG₁ monoclonal antibody and an IgG₂ monoclonal antibody.

3. A kit in accordance with claim 1 or 2 wherein said second monoclonal antibody reacts with substantially all monocytes.

4. A kit in accordance with claim 1 or 2 wherein said third monoclonal antibody reacts with substantially all T-cells and said fourth monoclonal antibodies reacts with substantially all B-cells.

5. A kit in accordance with claim 1 or 2 wherein said third monoclonal antibody reacts with substantially all T-cells and said fourth monoclonal antibody reacts with at least one activation antigen expressed by T-Cells.

6. A kit in accordance with claim 1 or 2 wherein said third monoclonal antibody reacts with substantially all CD4 bearing cells and said fourth monoclonal antibody reacts with substantially all cells expressing the Leu 8 antigen.

7. A kit in accordance with claim 1 or 2 wherein said third monoclonal antibody reacts with substantially all CD4 bearing cells and said fourth monoclonal antibody reacts with substantially all CD8 bearing cells.

8. A kit in accordance with claim 1 or 2 wherein said third monoclonal antibody reacts with substantially all CD8 bearing cells and said fourth monoclonal antibody reacts with substantially all cells expressing the Leu 7 antigen.