Electrophoresis pattern reading system of fluorescence type
First Claim
1. An electrophoresis pattern reading system of fluorescence-detection type, useful for analyzing a gel-based sample, the sample being labeled with a fluorescent substance that fluoresces upon application of light thereto, comprising:
- a detachable migration unit comprising a gel functioning as a base for a sample to be analyzed by electrophoresis and a gel-supporting body for supporting the gel;
an electrophoresis unit, to which the migration unit is detachably mounted, for performing electrophoresis by applying migrating voltage to the gel to which the sample labeled with a fluorescent substance is added; and
a reading unit physically separate from the electrophoresis unit for reading an electrophoresis pattern, the reading unit including means for detachably mounting the migration unit detached from and apart from the electrophoresis unit after electrophoresis, and said reading unit having means for passing light to the detachably mounted migration unit and for receiving fluorescence emitted from the fluorescent substance of the sample on the gel upon application of the light.
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Abstract
An electrophoresis pattern reading system of fluorescent type is comprised of a detachable migration unit comprising a gel functioning as a base for electrophoresis and a gel-supporting body for supporting the gel; an electrophoresis unit, to which the migration unit is mounted, for performing electrophoresis by applying migrating voltage to the gel to which a sample labeled with a fluorescent substance is added; and a reading unit including an instrumentation subunit for reading an electrophoresis pattern, to which the migration unit is mounted after electrophoresis and which receives fluorescence emitted from the fluorescent substance of the sample on the gel upon application of light to the gel. For example, a plurality of the migration units and the electrophoresis units are provided, and each of the plural migration units are mounted to one reading unit in order after electrophoresis has been performed with the respective electrophoresis units for a long period of time, thereby reading the electrophoresis pattern in a short period of time.
83 Citations
24 Claims
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1. An electrophoresis pattern reading system of fluorescence-detection type, useful for analyzing a gel-based sample, the sample being labeled with a fluorescent substance that fluoresces upon application of light thereto, comprising:
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a detachable migration unit comprising a gel functioning as a base for a sample to be analyzed by electrophoresis and a gel-supporting body for supporting the gel; an electrophoresis unit, to which the migration unit is detachably mounted, for performing electrophoresis by applying migrating voltage to the gel to which the sample labeled with a fluorescent substance is added; and a reading unit physically separate from the electrophoresis unit for reading an electrophoresis pattern, the reading unit including means for detachably mounting the migration unit detached from and apart from the electrophoresis unit after electrophoresis, and said reading unit having means for passing light to the detachably mounted migration unit and for receiving fluorescence emitted from the fluorescent substance of the sample on the gel upon application of the light. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A method for reading an electrophoresis pattern of fluorescence-detection type, comprising:
the step of pouring a sample into a gel in a migration unit, the step of mounting the migration unit in an electrophoresis unit, thereafter the step of performing electrophoresis of the sample, the step of thereafter removing the migration unit with the electrophoresed sample from the electrophoresis unit, the step of mounting the removed migration unit with the electrophoresed sample in a reading unit, and the step of reading the electrophoresis pattern of the electrophoresed sample in the migration unit by the reading unit. - View Dependent Claims (20, 21, 22, 23)
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24. A method for reading an electrophoresis pattern of fluorescence-detection type, comprising:
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(a) pouring a plurality of samples one each into a like plurality of gels one each into a like plurality of migration units; (b) mounting each migration unit one each in a like plurality of electrophoresis units; (c) thereafter performing electrophoresis of each sample; (d) thereafter removing one migration unit with its electrophoresed sample from a first one of the plurality of electrophoresis units; (e) mounting the removed migration unit with its electrophoresed sample in a reading unit; (f) reading the electrophoresis pattern of the electrophoresis sample in the migration unit by the reading unit; and (g) repeating the steps (d) through (f) for each remaining electrophoresed sample, and using the same reading unit for each reading step.
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Specification