Casting of gradient gels
First Claim
1. A method of casting a gradient slab gel comprising the steps of:
- filling a countable number of grooves in a first plate with a first gelling liquid;
forming a gel casting region for a slab gel by spacing said first plate apart from a second plate so as to provide a flow path between said first and second plates which has a directional component orthogonal to said grooves;
injecting a second gelling liquid into the gel casting region between said plates, such that said first liquid mixes with said second liquid as said second liquid encounters each said groove, forming a gelling mixture with a composition gradient;
causing said gelling mixture in the gel casting region to gel, thereby forming a gradient slab gel.
4 Assignments
0 Petitions
Accused Products
Abstract
A method and apparatus are presented for the casting of gradient gels, without voids, in confined support structures such as thin slab-type configurations. Furthermore, by using a differential activation scheme for the casting, the method makes is possible to produce such gels in slabs of capillary cross-sectional dimension. According to a preferred method, a gradient slab gel is cast by filling a countable number of grooves in a first plate with a first gelling liquid. A gel casting region for a slab gel is formed by spacing the first plate apart from a second plate so as to provide a flow path between the first and second plates which has a directional component orthogonal to the grooves. A second gelling liquid is injected into the gel casting region between the plates, such that the first liquid mixes with the second liquid as the second liquid encounters each groove, thereby forming a gelling mixture with a composition gradient. The gelling mixture is then caused to gel in the gel casting region, thereby forming a gradient slab gel.
158 Citations
22 Claims
-
1. A method of casting a gradient slab gel comprising the steps of:
-
filling a countable number of grooves in a first plate with a first gelling liquid; forming a gel casting region for a slab gel by spacing said first plate apart from a second plate so as to provide a flow path between said first and second plates which has a directional component orthogonal to said grooves; injecting a second gelling liquid into the gel casting region between said plates, such that said first liquid mixes with said second liquid as said second liquid encounters each said groove, forming a gelling mixture with a composition gradient; causing said gelling mixture in the gel casting region to gel, thereby forming a gradient slab gel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
-
-
11. Apparatus for casting a gel comprising:
-
a support structure for containing a gelling mixture that is promoted to gel by absorption of energy comprising; two substantially parallel plates that are spaced apart, at least one of said plates, hereinafter the first plate, constructed of a material that transmits energy; one or more substantially parallel edge seals positioned so as to define a liquid flow path between said plates; and a countable number of grooves situated on the surface of at least one of said plates wherein the grooves face the flow path; energy source means for providing energy at a localized area on said first plate; translation means for causing relative motion of said support structure and said energy source so that said localized area that receives energy from said source is moved along said first plate in a direction from the first end toward the second end of said first plate, thereby causing gelation to occur in said gelling mixture in a moving zone substantially adjacent to said localized area as said localized area moves. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19)
-
-
20. A gel support structure for casting a gradient slab gel comprising:
-
two substantially parallel plates that are spaced apart; one or more substantially parallel edge seals positioned so as to define a liquid flow path between said plates; and a countable number of grooves sitated on the surface of at least one of said plates wherein the grooves face the flow path, wherein said grooves are substantially linear and wherein said grooves are situated orthogonal to said edge seals.
-
-
21. A slab gel comprising:
-
a) a support structure comprising; two substantially parallel plates that are spaced apart by 1000 microns or less; and a countable number of grooves situated on the surface of at least one of said plates wherein the grooves situated on the surface at least one of said plates wherein the grooves face the flow path; and b) a gel disposed in the flow path and grooves, said gel having a composition that forms a concentration gradient from one end of the flow path to the other end. - View Dependent Claims (22)
-
Specification