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Casting of gradient gels

  • US 5,071,531 A
  • Filed: 05/01/1989
  • Issued: 12/10/1991
  • Est. Priority Date: 05/01/1989
  • Status: Expired due to Fees
First Claim
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1. A method of casting a gradient slab gel comprising the steps of:

  • filling a countable number of grooves in a first plate with a first gelling liquid;

    forming a gel casting region for a slab gel by spacing said first plate apart from a second plate so as to provide a flow path between said first and second plates which has a directional component orthogonal to said grooves;

    injecting a second gelling liquid into the gel casting region between said plates, such that said first liquid mixes with said second liquid as said second liquid encounters each said groove, forming a gelling mixture with a composition gradient;

    causing said gelling mixture in the gel casting region to gel, thereby forming a gradient slab gel.

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