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Test carrier for analysis of a sample liquid, method of using a test carrier and process for making a test carrier layer

  • US 5,071,746 A
  • Filed: 04/13/1989
  • Issued: 12/10/1991
  • Est. Priority Date: 04/28/1988
  • Status: Expired due to Fees
First Claim
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1. Test carrier useful in determination of an analyte in a liquid sample, comprising:

  • (i) a liquid absorbing first layer which contains a soluble conjugate of a first immunological binding partner and a label, the immunological binding partner of which specifically binds to said analyte to form a complex of analyte and conjugate;

    (ii) a porous second layer in permanent fluid communication with said first layer and containing a solid phase bound analyte or analyte analogue which binds to said first immunological binding partner but does not specifically bind with said analyte, wherein said second layer absorbs liquid at a rate which spreads liquid therethrough in an amount of time less than an amount of time necessary for completion of a binding reaction between said solid phase bound analyte or analyte analogue and any of first immunological binding partner not bound to analyte, and(iii) a third, liquid absorbing layer which contains a reagent which reacts with any conjugate of first immunological binding partner and label bound to said analyte to form an optically detectable signal, wherein said third layer is positioned on a flap means in said test carrier so that it is not in permanent contact with said second layer but may be brought into contact therewith as desired to permit liquid flow from said second layer to said third layer said contact permitting passage of liquid containing said complex of analyte and conjugate from said second layer into said third layer and penetration therein, to form said optically detectable signal.

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