Method and device for ketone measurement

• US 5,071,769 A
• Filed: 12/17/1987
• Issued: 12/10/1991
• Est. Priority Date: 12/22/1986
• Status: Expired due to Fees

• Current Assignee: Abbott Laboratories Incorporated
• Original Assignee: Abbott Laboratories Incorporated
• Inventors: Kundu, Samar K., March, Steven C., Rutnarak, Sangvorn, George, Richard W.
• Primary Examiner(s): WARDEN, JILL ALICE
• Application Number:
  US07/131,811
• Time in Patent Office: 1,454 Days
• Field of Search: 422/83, 422/84, 422/56-58, 422/61, 422/101, 436/128, 436/130, 436/165, 436/167, 436/169, 436/170, 436/177, 436/178
• US Class Current: 436/128
• CPC Class Codes: A61B 5/083 Measuring rate of metabolism by using breath test, e.g. measuring rate of oxygen consumption
• CPC Class Codes: A61B 5/4872 Body fat
• CPC Class Codes: G01N 31/223 for investigating presence of specific gases or aerosols G01N31/221, G01N31/222 take precedence; actuation of fire alarm by presence of smoke or gases G08B17/10
• CPC Class Codes: G01N 33/497 of gaseous biological material, e.g. breath
• CPC Class Codes: G01N 33/64 involving ketones
• CPC Class Codes: G01N 33/98 involving alcohol, e.g. ethanol in breath
• CPC Class Codes: Y10T 436/200833 Carbonyl, ether, aldehyde or ketone containing
• CPC Class Codes: Y10T 436/202499 Formaldehyde or acetone
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

Methods and materials detect ketone and aldehyde analytes in fluid samples by reacting analyte containing samples with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which an amine is covalently coupled. Methods and devices are also provided for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising determining the breath acetone concentration of the subject.

Claims

1. A method for ascertaining the fat catabolism effects of a weight loss dietary regimen, said method comprising:

(a) periodically assaying breath for acetone content, and

(b) correlating breath acetone content to a standard reflecting the effect on breath acetone of fixed rates of fat catabolism.

2. The method according to claim 1 wherein the period is 24 hours and wherein the fixed rate is expressed in ounces of fat catabolized per day.

3. The method according to claim 1 wherein the standard is in the form of an equation.

4. The method according to claim 1 wherein the standard is in tabular form.

5. The method according to claim 1 wherein the standard is a graphic adjunct to a breath acetone assaying device.

6. The method for ascertaining the fat catabolism effect of a weight loss dietary regimen according to claim 1 wherein the breath is assayed for acetone content by the steps of:

(i) contacting a breath sample with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which a primary or secondary amine is covalently coupled, (ii) reacting the acetone with the nitroprusside and amine to form a detectable reaction product, and (iii) detecting the reaction product.

7. A kit for the determination of ketone and aldehyde concentrations in a vapor sample comprising:

(a) a first solid matrix material to which a nitroprusside salt is coupled,

(b) a second solid matrix material to which a primary or secondary amine is covalently bound,

(c) a solvent, and

(d) means for collecting a fixed volume of sample vapor and contacting it with the first solid matrix material and the second solid matrix material.

8. A kit for the determination of ketone and aldehyde concentrations in a liquid sample comprising:

(a) a first solid matrix material to which a nitroprusside salt is coupled,

(b) a second solid matrix material to which a primary or secondary amine is covalently bound, and

(c) means for collecting a fixed volume of the liquid sample and contacting it with the first solid matrix material and the second solid matrix material.

9. A kit for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising:

(a) a first solid matrix material to which a nitroprusside salt is coupled,

(b) a second solid matrix material to which a primary or secondary amine is covalently bound,

(c) a solvent,

(d) means for collecting a fixed volume of breath and contacting it with the first solid matrix material and the second solid matrix material, and

(e) a standard reflecting the effect on breath acetone of fixed rates of fat catabolism.