Test card for performing assays
First Claim
1. Apparatus for supporting a plurality of selected capture reagents, each reactive with a specific assay binding component of interest in a biological sample, for use in simultaneously assaying the sample for said assay binding components, comprising:
- A first layer of capture reagent binding material;
a second layer of non-absorbent substrate material below the first layer of capture reagent binding material;
a plurality of test sites formed in said layer of capture reagent binding material adapted to bind a selected capture reagent; and
isolation means for isolating each test site from every other test site and for confining capture reagent applied to one of said test sites, wherein said isolation means includes a plurality of isolation moats disposed about said test sites and extending through said first layer of capture reagent binding material and partially into said second layer of non-absorbent substrate material.
1 Assignment
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Accused Products
Abstract
A semi-automated biological sample analyzer and subsystems are provided to simultaneously perform a plurality of enzyme immuno assays for human IgE class antibodies specific to a panel of preselected allergens in each of a plurality of biological samples. A carousel is provided to position and hold a plurality of reaction cartridges. Each reaction cartridge includes a plurality of isolated test sites formed in a two dimensional array in a solid phase binding layer contained within a reaction well which is adapted to contain a biological sample to be assayed. The carousel and cartridges contain structures which cooperate to precisely position the cartridges in each of three separate dimensions so that each cartridge is positioned uniformly. An optical reader operating on a principle of diffuse reflectance is provided to read the results of the assays from each test site of each cartridge. Also provided is a subsystem which provides predetermined lot-specific assay calibration data which is useful for normalizing the results of various assays with respect to predetermined common standard values.
283 Citations
24 Claims
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1. Apparatus for supporting a plurality of selected capture reagents, each reactive with a specific assay binding component of interest in a biological sample, for use in simultaneously assaying the sample for said assay binding components, comprising:
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A first layer of capture reagent binding material; a second layer of non-absorbent substrate material below the first layer of capture reagent binding material; a plurality of test sites formed in said layer of capture reagent binding material adapted to bind a selected capture reagent; and isolation means for isolating each test site from every other test site and for confining capture reagent applied to one of said test sites, wherein said isolation means includes a plurality of isolation moats disposed about said test sites and extending through said first layer of capture reagent binding material and partially into said second layer of non-absorbent substrate material. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. An apparatus for supporting a plurality of selected capture reagents, each reactive with a specific assay binding component of interest in a biological sample, for use in simultaneously assaying the sample for said assay binding components, comprising:
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a polyester film substrate; a layer of a capture reagent binding material mounted to said polyester film substrate, said capture reagent binding material selected from the group consisting of nitrocellulose and nylon; an array of test sites formed in said layer of capture reagent binding material, each test site having a uniform diameter and a predetermined volume; and a plurality of isolation moats formed in and extending through said layer of capture reagent binding material and partially into said polyester film substrate;
wherein said isolation moats are for isolating said test site and for confining capture reagent applied to one of said test sites to said predetermined volume of said one of said test sites.
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13. An apparatus for use in assaying a biological sample for a plurality of assay binding components, comprising:
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a first layer of capture reagent binding material; a second layer of non-absorbent substrate material below the first layer of capture reagent binding material; a plurality of test sites formed in said layer of capture reagent binding material, each having bound thereon a selected capture reagent which is reactive with a specific assay binding component of a biological sample; and isolation means for isolating each test site and for confining capture reagent applied to one of said test sites, wherein said isolation means includes a plurality of isolation moats formed in said first layer of capture reagent binding material disposed about said test sites and extending through said layer of capture reagent binding material and partially into the second layer of non-absorbent substrate material. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20)
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21. An apparatus for supporting a plurality of selected capture reagent, each capture reagent being reactive with a specific assay binding component of interest in a biological sample, for use in simultaneously assaying the sample for said assay binding components, comprising:
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a first layer of capture reagent binding material; a second layer of hydrophobic material below the first layer of capture reagent binding material; a third layer of non-absorbent substrate material below the second layer of hydrophobic material; a plurality of test sites formed in the first layer of capture reagent binding material, each adapted to bind a selected capture reagent; and isolation means for isolating each test site from every other test site and for confining capture reagent applied to one of said test sites to each said test site, wherein said isolation means include a plurality of isolation moats disposed about said test sites and extending through said first layer and partially or completely through the second layer. - View Dependent Claims (22, 23, 24)
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Specification