Slide analysis system

• US 5,075,079 A
• Filed: 05/21/1990
• Issued: 12/24/1991
• Est. Priority Date: 05/21/1990
• Status: Expired due to Fees

• Current Assignee: Technicon Instruments Corporation (Bayer AG)
• Original Assignee: Technicon Instruments Corporation (Bayer AG)
• Inventors: Mernyk, Edwin H., Kerr, Alexander F., Zabetakis, George E., Escoli, Uri
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Trautman, Kimberly A.
• Application Number:
  US07/526,270
• Time in Patent Office: 582 Days
• Field of Search: 422/63, 422/64, 422/65, 422/67, 422/68.1, 436/43, 436/45, 436/47, 436/49, 73/863.11, 73/864.21
• US Class Current: 422/64
• CPC Class Codes: G01J 3/2803 using photoelectric array detector
• CPC Class Codes: G01N 2035/00039 Transport arrangements specific to flat sample substrates, e.g. pusher blade
• CPC Class Codes: G01N 2035/00089 Magazines
• CPC Class Codes: G01N 2035/00138 Slides
• CPC Class Codes: G01N 2035/00356 Holding samples at elevated temperature (incubation)
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 2035/103 using disposable tips
• CPC Class Codes: G01N 2035/1086 Cylindrical, e.g. variable angle
• CPC Class Codes: G01N 2201/082 Fibres for a reference path
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/114998 with treatment or replacement of aspirator element [e.g., cleaning, etc.]

Abstract

The slide analysis system includes a slide holding module spaced from an incubator module. A slide transfer device or pick and place mechanism withdraws slides from the slide holding module and inserts them into the incubator module. A metering device deposits serum onto a slide that is held in a spotting position by the slide transfer device. The metering device is movable to a sampling position in the slide holding module to aspirate serum from a serum source on the slide cartridge in the slide holding module and is also movable to a spotting position to spot serum on the slide held by the slide transfer device. The slide transfer device has rotational as well as transverse movement and includes jaws for gripping onto the sides of slides to withdraw a single slide from a cartridge, transport it to the incubator, insert the slide in the incubator and thereafter remove it from the incubator. The metering device also has rotational as well as elevational movement and includes a built-in pipette tip ejector for discarding a used pipette tip.

Claims

1. A slide analysis system adapted to measure a characteristic of a sample fluid deposited on an analysis slide comprising:

a) slide holding means for holding a plurality of slides to be used in the analysis;

b) incubator means including a a plurality of slide holders, spaced a predetermined distance from said slide holding means;

c) depositing means spaced from said slide holding means and said incubator means for depositing a sample fluid on selected ones of the slides to provide spotted slides;

d) transfer means for withdrawing individual ones of the slides from said slide holding means and for inserting the individually withdrawn slides in said incubator means, said transfer means including;

i) slide engaging means;

ii) means for rotating said slide engaging means to a first predetermined rotational position to align said slide engaging means with one of the slides in said slide holding means, and a second predetermined rotational position to align said slide engaging means with one of said slide holders in said incubator means; and

iii) means for displacing said slide engaging means when in said first predetermined rotational position to a first predetermined displaced position at said slide holding means to engage said one of the slides in said slide holding means and for displacing said slide engaging means away from said slide holding means with said one of the slides, and when said slide engaging means is in said second predetermined rotational position to a second predetermined displaced position at said incubator means to insert a slide in said one of said slide holders or remove a slide from said one of said slide holders and for displacing said slide engaging means, with or without a slide, away from said one of said slide holders.

2. The slide analysis system as claimed in claim 1 wherein said slide engaging means includes a pair of elongated gripper members pivoted together to permit pivotal movement toward and away from each other, said gripper members having a first converged position for gripping a slide, and a second diverged position for releasing the grip on the slide.

3. The slide analysis system as claimed in claim 2 further including biasing means cooperable with said gripper members to normally urge said gripper members to said first converted position.

4. The slide analysis system as claimed in claim 2 further including spacer means for causing said gripper to diverge from said first converged position to said second diverged position.

5. The slide analysis system as claimed in claim 1 wherein said slide engaging means includes a pair of separable gripper members for engaging and gripping opposite sides of the slides.

6. The slide analysis system as claimed in claim 5 wherein said slide engaging means include means for converging said gripper members to engage and grip the opposite sides of the slides.

7. The slide analysis system as claimed in claim 6 wherein said slide engaging means further include biasing means cooperating with said gripper members for normally urging said gripper members to converge.

8. The slide analysis system as claimed in claim 5 wherein said gripper members include means for supporting a slide.

9. The slide analysis system as claimed in claim 5 wherein said gripper members include a pair of elongated jaws pivoted together to permit pivotal movement toward and away from each other.

10. The slide analysis system as claimed in claim 1 including means for coordinating the movement of said rotating means and said displacing means such that said rotating means and said displacing means are separately or simultaneously operable.

11. The slide analysis system as claimed in claim 1 wherein said rotating means include means for rotating said slide engaging means to a third predetermined rotational position to permit spotting of a slide held in said slide engaging means.

12. A slide analysis system adapted to measure a characteristic of a sample fluid deposited on an analysis slide comprising:

a) a slide holding means for holding a plurality of slides to be used in the analysis;

b) incubator means for the slides, spaced a predetermined distance from said slide holdings means;

c) depositing means spaced from said slide holding means and said incubator means for depositing sample fluid on selected ones of the slides to provide spotted slides;

d) transfer means for transferring slides from said slide holding means to said incubator means, said slide holding means and said incubator means located at predetermined angular positions with respect to said transfer means, said transfer means including;

i) slide engaging means; and

ii) moving means for rotating said slide engaging means between said predetermined angular positions of said slide holding means and said incubator means; and

iii) displacing means for displacing said slide engaging means forwardly and rearwardly in the direction defined by said predetermined angular positions.

13. The slide analysis system as claimed in claim 12 including means for coordinating the movement of said moving means and said displacing means such that said moving means and said displacing means are separately or simultaneously operable.

14. The slide analysis system as claimed in claim 12 wherein said moving means include means for rotating said slide engaging means to a third predetermined angular position to permit spotting of a slide held in said slide engaging means.

15. The slide analysis system as claimed in claim 12 wherein said slide engaging means includes a pair of elongated gripper members pivoted together to permit pivotal movement toward and away from each other, said gripper members having a first converged position for gripping a slide, and a second diverged position for releasing the grip on the slide.

16. The slide analysis system as claimed in claim 15 further including biasing means cooperable with said gripping means to normally urge said gripper members to said first converged position.

17. The slide analysis system as claimed in claim 15 further including spacer means for causing said grippers to diverge from said first converged position to said second diverged position.