Rapid stick test for the diagnosis of bovine leukemia virus infection from serum or milk
First Claim
1. A test kit for the detection of antibodies to bovine leukemia virus in cow'"'"'s milk which comprises as individual components:
- (a) a solid phase support dipstick having attached thereon at least two-spaced apart suitable antigen-binding substances;
a first of said antigen-binding substances having bound thereon one or more bovine leukemia virus antigens capable of forming immuno-complexes with the antibodies which may be present in the specimen, anda second of said antigen-binding substances having bound thereon one or more control antigens for the detection of non-specific background;
(b) a series of five sealed containers with removable closures containing in series;
(i) an empty sterile container,(ii) a first wash solution,(iii) a solution containing enzyme conjugated to a second-antibody, said second-antibody being capable of forming an immunocomplex with the specimen antibodies,(iv) a second wash solution,(v) a substrate which reacts chemically with said enzymes so as to form a colored reaction product thereby indicating the presence of anti-viral antibodies in said specimen;
said dipstick being attached to a removable closure of one of said containers.
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Accused Products
Abstract
A method for the detection of the presence of anti-viral or anti-bacterial antibodies in a specimen and a kit for the same. The method comprises the use of a solid phase support dipstick having attached thereto suitable antigen-binding material to which viral antigens or bacterial antigens are bound. The antibodies specific for the bound antigens bind to the antigen and a second-antibody labelled with a means for visually detecting those antibodies which have formed immunocomplexes with the bound nitrogen when in the presence of suitable reactive agents. Also, a method of using such a dipstick in this method and in the test kit is disclosed. Specifically, boving leukemia virus is tested for; a dipstick attached to a removable closure is employed; and the dipstick has two antigen binding areas, one of which is a control.
13 Citations
20 Claims
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1. A test kit for the detection of antibodies to bovine leukemia virus in cow'"'"'s milk which comprises as individual components:
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(a) a solid phase support dipstick having attached thereon at least two-spaced apart suitable antigen-binding substances; a first of said antigen-binding substances having bound thereon one or more bovine leukemia virus antigens capable of forming immuno-complexes with the antibodies which may be present in the specimen, and a second of said antigen-binding substances having bound thereon one or more control antigens for the detection of non-specific background; (b) a series of five sealed containers with removable closures containing in series; (i) an empty sterile container, (ii) a first wash solution, (iii) a solution containing enzyme conjugated to a second-antibody, said second-antibody being capable of forming an immunocomplex with the specimen antibodies, (iv) a second wash solution, (v) a substrate which reacts chemically with said enzymes so as to form a colored reaction product thereby indicating the presence of anti-viral antibodies in said specimen; said dipstick being attached to a removable closure of one of said containers. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification