Ready to use liquid reagent for determining the glucose content in blood

• US 5,077,199 A
• Filed: 01/03/1985
• Issued: 12/31/1991
• Est. Priority Date: 01/27/1984
• Status: Expired due to Fees

• Current Assignee: A Menarini Industrie Farmaceutiche Riunite SRL
• Original Assignee: A Menarini SAS
• Inventors: Basagni, Umberto, Bonicolini, Francesco
• Primary Examiner(s): Kepplinger, Esther M.
• Application Number:
  US06/688,587
• Time in Patent Office: 2,553 Days
• Field of Search: 435/14, 435/25, 435/28, 435/190, 435/810, 435/814, 435/188
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

Stable and ready to use liquid reagent for determining the glucose content in blood, comprising a glucose-oxidase free from catalase and a nonionic surface-active agent, such as, for example, the polyoxyethylene derivatives, hydroxypolyethylene ethoxydodecane, the lauryl ether of polyoxyethylene-glycol, as a stabilizer.

Claims

1. A ready-to-use aqueous liquid reagent for colorimetric determination of the glucose content in blood, comprising:

(a) from 9,000 to 40,000 u.i/liter of enzymatically-active glucose oxidase free from catalase; and

(b) from 5 mg/liter to 50 g/liter of a nonionic surface active agent effective to stabilize the liquid reagent.

2. A liquid reagent according to claim 1, wherein the surface active agent is a polyoxyethylene derivative.

3. A liquid reagent according to claim 2, wherein the surface active agent is polyoxyethylene-lauryl alcohol.

4. A liquid reagent according to claim 2, wherein the surface active agent is polyoxyethylene-octylphenol.

5. A liquid reagent according to claim 1, further comprising peroxidase and a chromogenic system such that upon combination of the reagent with a glucose-containing sample a colored product will be formed.

6. A liquid reagent according to claim 5, wherein the chromogenic system comprises phenol and 4-aminophenazone or a derivative thereof.

7. A liquid reagent according to claim 5, wherein the chromogenic system comprises phenol and 4-aminoantipyrine.