Neutralized perfluoro-3,6-dioxa-4-methyl-7-octene sulphonyl fluoride copolymer surface for attachment and growth of animal cells

• US 5,077,215 A
• Filed: 07/17/1989
• Issued: 12/31/1991
• Est. Priority Date: 09/17/1987
• Status: Expired due to Fees

• Current Assignee: Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education), Telectronics Incorporated
• Original Assignee: Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education), Telectronics Incorporated
• Inventors: Norris, William, McAuslan, Brian R., Johnson, Graham, Steele, John G.
• Primary Examiner(s): Stone, Jacqueline
• Assistant Examiner(s): Elliott, George C.
• Application Number:
  US07/360,887
• Time in Patent Office: 897 Days
• Field of Search: 435/240.23, 435/240, 435/243, 600/36, 623/1, 424/423
• US Class Current: 424/423
• CPC Class Codes: C12N 2533/30 Synthetic polymers
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: Y10S 623/915 Method or apparatus for preparing biological material
• CPC Class Codes: Y10S 623/92 Method or apparatus for preparing or treating prosthetic

Abstract

Neutralized surface of a polymer of perfluoro-3,6-dioxa-4-methyl-7-octene sulphonyl fluoride for attachment and growth of animal cells in vivo or in vitro, the comonomer preferably being tetrfluorethylene.

Claims

1. In a prosthesis or sponge implantable in a body, the improvement comprising forming the surface of said prosthesis or sponge from a neutralized copolymer of perfluoro-3,6-dioxa-4-methyl-7-octene sulphonyl fluoride and a monomer.

2. The prosthesis or sponge of claim 1 wherein the monomer is tetrafluoroethylene.

3. The prosthesis or sponge of claim 1 further comprising a supporting material to which said copolymer is applied.

4. The prosthesis or sponge of claim 3 wherein said supporting material is selected from the group consisting of a polymer, ceramic, metal, glass and preformed membrane.

5. The prosthesis or sponge of claim 4 wherein said polymer is a porous polymer.

6. The prosthesis or sponge of claim 5 wherein said porous polymer is polytetrafluoroethylene or expanded polytetrafluoroethylene.

7. The prosthesis or sponge of claim 5 wherein the porous polymer is knitted or woven polyester.

8. The prosthesis or sponge of claim 5 wherein the porous polymer is polyurethane.

9. The prosthesis of sponge of claim 1 in the form of a tube.

10. The prosthesis or sponge of claim 1 wherein the surface further comprises adsorbed adhesive proteins.

11. The prosthesis or sponge of claim 10 wherein said adhesive proteins are derived from serum.

12. The prosthesis or sponge of claim 11 wherein said adhesive serum proteins are selected from the group consisting of fibronectin, vitronectin, thrombospondin and adhesive fragments of any of these proteins.

13. The prosthesis or sponge of claim 1 wherein the surface further comprises animal cells adhered thereto.

14. A surface for the attachment and growth of animal cells in vivo, said surface comprising the neutralized form of a copolymer of perfluoro-3,6-dioxa-4-methyl-7-octene sulphonyl fluoride and monomer with animal cells adhered thereto.

15. A process for the preparation of a surface for the attachment and growth of animal cells in vivo, said process comprising applying a copolymer of perfluoro-3-, 6-dioxa-4-methyl-7-octene sulphonyl fluoride and a monomer to an appropriate substrate, neutralizing the resultant surface and adhering animal cells to the surface.

16. A process for the attachment and growth of animal cells in vivo comprising:

exposing animal cells to a prosthesis or sponge having a surface formed from a neutralized copolymer of perfluoro-3,6-dioxa-4-methyl-7-octene sulphonyl fluoride and a monomer.

17. A process for the attachment and growth of animal cells in vitro comprising:

exposing animal cells in vitro to a surface formed from a neutralized copolymer of perfluoro-3,6-dioxa-4-methyl-7-octene sulphonyl fluoride and a monomer.

18. The process of claim 17 which further comprises exposing said surface to a medium containing animal cells and adhesive proteins.