Method for producing vitronectin

• US 5,077,393 A
• Filed: 03/22/1988
• Issued: 12/31/1991
• Est. Priority Date: 05/25/1987
• Status: Expired due to Term

• Current Assignee: Research Development Corporation Of Japan (Japan Science And Technology Agency)
• Original Assignee: Research Development Corporation Of Japan (Japan Science And Technology Agency)
• Inventors: Hayashi, Masao
• Primary Examiner(s): Wax, Robert A.
• Assistant Examiner(s): Marschel, Ardin
• Application Number:
  US07/171,645
• Time in Patent Office: 1,379 Days
• Field of Search: 530/350, 530/380, 530/395, 530/412, 530/415, 530/417, 530/419, 530/413, 435/101, 435/103, 935/9
• US Class Current: 530/413
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: C07K 14/78 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: G06N 3/002 Biomolecular computers, i.e. using biomolecules, proteins, cells using DNA G06N3/123; using neurons G06N3/061

Abstract

This invention deals with a simple and efficient method for producing vitronectin from biological materials which include vitronectin. More specifically, this invention deals with a method for producing vitronectin by binding vitronectin in biological materials to immobilized glycosaminoglycans in the presence of protein denaturing agent, especially urea.

Claims

1. A method for recovering purified vitronectin from a biological material in which vitronectin is present, which consists essentially of contacting immobilized glycosaminoglycan with the biological material in the presence of a protein denaturing agent whereby the conformation of vitronectin present in the biological material is altered such that the binding activity of said vitronectin to glycosaminoglycan is selectivity increased whereby vitronectin binds selectively to the immobilized glycosaminoglycan and recovering the bound vitronectin.

2. The method of claim 1 wherein the glycosaminoglycan comprises heparin and the protein denaturing agent comprises urea.

3. The method of claim 1 wherein the glycosaminoglycan comprises heparin and the protein denaturing agent comprises quanidine-HCl.

4. The method of claim 1 wherein the biological material in which vitronectin is present comprises animal serum, body fluid, tissues or their processed substances.

5. The method of claim 1 wherein the biological material in which vitronectin is present comprises animal plasma.

6. The method of claim 1 wherein the biological material containing vitronectin comprises tissue-culture cells, cell culture medium or processed substances derived from tissue culture cells or cell culture medium.

7. The method of claim 2 wherein the urea is added at a concentration of from about 4 to 8 molar.

8. The method of claim 2 wherein the urea is added at a concentration of about 8M.