Apparatus for in vivo analysis of biological compounds in blood or tissue by microdialysis and mass spectrometry

• US 5,078,135 A
• Filed: 05/31/1989
• Issued: 01/07/1992
• Est. Priority Date: 05/31/1989
• Status: Expired due to Fees

• Current Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Inventors: Caprioli, Richard M.
• Primary Examiner(s): Smith, Ruth S.
• Application Number:
  US07/359,475
• Time in Patent Office: 951 Days
• Field of Search: 128/632, 128/635, 128/637, 604/4-6, 604/50-53, 604/65-67
• US Class Current: 600/309
• CPC Class Codes: A61B 5/14528 invasively
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/14542 for measuring blood gases A61B5/14551 takes precedence
• CPC Class Codes: A61B 5/1473 invasive, e.g. introduced into the body by a catheter A61B5/1482 takes precedence
• CPC Class Codes: A61B 5/412 Detecting or monitoring sepsis

Abstract

The present invention combines microdialysis with mass spectrometry, for example continuous flow fast atom bombardment, to follow the pharmacokinetics of drugs or other compounds directly in the blood stream or tissues of a live animal. After intramuscular injection of the drug, the blood dialysate from a microdialysis probe inserted into a blood vessel or tissue of the animal, is allowed to flow into the mass spectrometer via the continuous flow fast atom bombardment interface. Tandem mass spectrometry allows for isolating and recording the ion fragments produced from the drug as the dialysate is exposed to the ionization process. The detected concentration of the drug or other compounds of interest can be used to adjust the rate of administration of the drug.

Claims

1. An apparatus for measuring specific biological compounds in a living animal, comprising:

an implantable microdialysis probe having a channel with a perfusate input and a dialysate output, said channel including a dialyzing membrane;

a continuous source of perfusate connected to said perfusate input;

mass spectrometer means for measuring an amount of specific biological compounds in said dialysate; and

switch valve means, connected between said dialysate output and said mass spectrometer means, positionable in a first position to collect dialysate and positionable in a second position to transfer collected dialysate to said mass spectrometer means.

2. The apparatus of claim 1, further comprising:

means, connected to said mass spectrometer means, for administering a drug to a live animal according to said amount of said specific biological compounds in said dialysate.

3. The apparatus of claim 1, wherein said mass spectrometer means is a continuous flow fast atom bombardment mass spectrometer.