Orthogonal flow immunoassays and devices
First Claim
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1. A device for the detection of an analyte comprising:
- a matrix in a first plane providing for capillary transport of an aqueous sample medium;
cover means positioned over said matrix, a first port in said cover means, with said first port positioned over said matrix for directing said sample medium to a first site on said matrix;
flow means in contact with said matrix at a second site on said matrix for directing flow of said sample medium at said second site to a second plane substantially parallel to said matrix in said first plane, said flow through said flow means being substantially perpendicular to said matrix;
a immunoglobulin bound to said matrix at said second site; and
absorbant means, in contact with said flow means, for receiving said sample medium in said second plane.
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Abstract
Methods and composition are provided for diagnostic assays, where a sample traverses a bibulous matrix in a first plane and the flow path redirected into a second plane at a binding site, where a signal is created in relation to the presence of analyte. Various protocols may be employed where components of a signal producing system may be combined with the sample, the matrix, or added at the binding site.
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Citations
13 Claims
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1. A device for the detection of an analyte comprising:
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a matrix in a first plane providing for capillary transport of an aqueous sample medium; cover means positioned over said matrix, a first port in said cover means, with said first port positioned over said matrix for directing said sample medium to a first site on said matrix; flow means in contact with said matrix at a second site on said matrix for directing flow of said sample medium at said second site to a second plane substantially parallel to said matrix in said first plane, said flow through said flow means being substantially perpendicular to said matrix; a immunoglobulin bound to said matrix at said second site; and absorbant means, in contact with said flow means, for receiving said sample medium in said second plane. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A multiunit device for the detection of at least one analyte comprising a plurality of containers, each container sharing at least one common wall with an adjacent container, each container comprising:
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a matrix in a first plane providing for capillary transport of an aqueous sample medium; cover means positioned over said matrix, a first port in said cover means, with said first port positioned over said matrix for direction said sample medium to a first site on said matrix; flow means in contact with said matrix at a second site on said matrix for directing flow of said sample medium at said second site to a second plane substantially parallel to said matrix in said first plane, said flow through said flow means being substantially perpendicular to said matrix; an immunoglobulin bound to said matrix at said second site; and absorbant means, in contact with said flow means, for receiving said sample medium in said second plane.
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10. A device for the detection of an analyte comprising:
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a container including a cover; a bibulous matrix in a first plane providing for capillary transport of an aqueous sample medium, said bibulous matrix in said container and under said cover; a first port in said cover, said first port positioned over said bibulous matrix for directing said sample medium to a first site on said matrix; flow means in contact with said matrix at a second site on said matrix for directing flow of said sample at said second site to a second plane substantially parallel to said bibulous matrix in said first plane, said flow through said flow means being substantially perpendicular to said bibulous matrix; an immunoglobulin bound to said bibulous matrix at said second site; and absorbant means, in contact with said flow means, for receiving said sample medium in said second plane. - View Dependent Claims (11, 12, 13)
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Specification