Fluorocarbon polymer support for chromatographic separations, diagnostic assays and enzyme immobilization
First Claim
Patent Images
1. A solid support containing an attached ligand or binder for the ligand consisting essentially of:
- (A) a non-perfluorocarbon solid carrier core;
(B) a fluorocarbon layer coated on said solid carrier core;
(C) a ligand or binder for the ligand securely but reversibly attached to the surface of said fluorocarbon layer through a reactive perfluorocarbon anchor compound; and
(D) a nonionic fluorosurfactant coating on said fluorocarbon layer.
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Abstract
A solid support useful for bioaffinity and ion-exchange separations and enzyme immobilization is provided. The support is based on a non-perfluorocarbon solid carrier core coated with a nonionic fluorosurfactant-coated fluorocarbon interlayer to which a ligand or a binder for the ligand is securely, but reversibly attached through a reactive perfluorocarbon anchor compound. Also provided is a solid support useful for size exclusion separations. Such support is based on a non-perfluorocarbon carrier core coated with a nonionic fluorosurfactant-coated fluorocarbon interlayer.
74 Citations
11 Claims
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1. A solid support containing an attached ligand or binder for the ligand consisting essentially of:
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(A) a non-perfluorocarbon solid carrier core; (B) a fluorocarbon layer coated on said solid carrier core; (C) a ligand or binder for the ligand securely but reversibly attached to the surface of said fluorocarbon layer through a reactive perfluorocarbon anchor compound; and (D) a nonionic fluorosurfactant coating on said fluorocarbon layer. - View Dependent Claims (2, 3)
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4. An ion-exchange chromatographic solid support consisting essentially of:
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(A) a non-perfluorocarbon solid carrier core; (B) a fluorocarbon layer coated on said solid carrier core; (C) a cationic fluorosurfactant securely but reversibly attached to the surface of said fluorocarbon layer; and (D) a nonionic fluorosurfactant coating on said fluorocarbon layer.
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5. A process of preparing a solid support containing an attached ligand or binder for the ligand comprising the steps of:
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(A) contacting a non-perfluorocarbon solid carrier core with a fluorocarbon compound to form a fluorocarbon layer on said solid carrier core; (B) modifying a ligand or binder for the ligand by attaching a reactive perfluorocarbon anchor compound to said ligand or binder for the ligand; (C) contacting said fluorocarbon layer on said solid carrier core with said perfluorocarbon-substituted ligand or binder for the ligand to attach the ligand or binder to the surface of said fluorocarbon layer through said reactive perfluorocarbon anchor compound; and (D) contacting said fluorocarbon layer with a nonionic fluorosurfactant.
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6. A process of preparing a solid support containing an attached ligand or binder for the ligand comprising the steps of:
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(A) contacting a non-perfluorocarbon solid carrier core with a fluorocarbon compound to form a fluorocarbon layer on said solid carrier core; (B) preactivating said fluorocarbon layer on said solid carrier core by attaching a reactive perfluorocarbon anchor compound to said fluorocarbon layer; (C) contacting said preactivated fluorocarbon layer on said solid carrier core with a ligand or binder for the ligand to attach said ligand or binder to the surface of said preactivated fluorocarbon layer through said reactive perfluorocarbon anchor compound; and (D) contacting said preactivated fluorocarbon layer with a nonionic fluorosurfactant. - View Dependent Claims (7, 8)
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9. The process of claim wherein said spacer group is a derivative of ethylene glycol.
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10. A bioaffinity separation process comprising the steps of:
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(A) forming a solid affinity support by (a) attaching a ligand or a binder for the ligand to the surface of a fluorocarbon layer coated on a non-perfluorocarbon solid carrier core through a reactive perfluorocarbon anchor compound; and (b) contacting the product of step (a) with a nonionic fluorosurfactant to form an affinity support; and (B) capturing a binder or ligand, complementary to the ligand or binder attached to said fluorocarbon layer from a mixture using said solid affinity support.
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11. An immobilized enzyme system consisting essentially of:
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(A) a non-perfluorocarbon solid carrier core; (B) a fluorocarbon layer coated on said solid carrier core; (C) an enzyme attached securely but reversibly to the surface of said fluorocarbon layer through a reactive perfluorocarbon anchor compound; and (D) a nonionic fluorosurfactant coating on said fluorocarbon layer.
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Specification