Waveguide sensor with input and reflecting gratings and its use in immunoassay

• US 5,081,012 A
• Filed: 01/22/1990
• Issued: 01/14/1992
• Est. Priority Date: 03/29/1988
• Status: Expired due to Term

• Current Assignee: Applied Research Systems ARS Holding NV (Merck & Co., Inc.)
• Original Assignee: Applied Research Systems ARS Holding NV (Merck & Co., Inc.)
• Inventors: Flanagan, Michael T., Sloper, Andrew N.
• Primary Examiner(s): Lacey, David l.
• Assistant Examiner(s): CHAN, KWONG
• Application Number:
  US07/435,519
• Time in Patent Office: 722 Days
• Field of Search: 356/128, 356/73.1, 435/291, 435/288, 435/7.1, 435/7.9, 435/7.92, 435/7.93, 435/7.94, 422/59, 422/82.09, 422/82.11, 436/172, 436/518, 436/524, 436/582
• US Class Current: 435/7.9
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B

Abstract

Waveguides suitable for use in optical assay techniques are described, as well as methods of assay employing such waveguides. The waveguide comprises an input grating structure to couple excitation radiation into the waveguide, a reflecting grating structure to reflect the excitation radiation propagating within the waveguide, and a transduction region located between the two grating structures, whereby during use the excitation radiation traverses the transduction region at least twice.

Claims

1. A planar waveguide comprising an input grating structure constructed so as to couple excitation radiation into the waveguide so as to propagate therethrough, a reflecting grating structure spacially disposed from said input grating structure and constructed so as to reflect said excitation radiation propagating within the waveguide, and a transduction region located between said input grating structure and said reflecting grating structure, whereby during use said excitation radiation traverses said transduction region at least twice.

2. A waveguide according to claim 1 having a thickness of 0.2 to 10.0 microns.

3. A waveguide according to claim 1 further comprising an output grating structure constructed so as to couple signal radiation out of the waveguide, whereby during use said signal radiation results from excitation of the transduction region and has a wavelength different from said excitation radiation.

4. A waveguide according to claim 3 further comprising a second reflecting grating structure constructed so as to reflect said signal radiation propagating within the waveguide.

5. A waveguide according to claim 1 which is substantially circular with a plurality of said input and reflecting grating structures and said transduction regions disposed concentrically.

6. A waveguide according to claim 1 further comprising at least one grating structure constructed so as to be able to filter, focus, defocus or collimate radiation.

7. A waveguide according to claim 1 wherein said transduction region has immobilized thereon, directly or indirectly, a material capable of specifically binding to a species to be assayed.

8. A waveguide sensor useful for the optical assay of a chemical, biological or biochemical substance, said waveguide sensor comprising a planar waveguide comprising an input grating structure constructed so as to couple excitation radiation into the waveguide so as to propagate therethrough, a reflecting grating structure spacially disposed from said input grating structure and constructed so as to reflect said excitation radiation propagating within the waveguide, and a transduction region located between said input grating structure and said reflecting grating structure, said transduction region having immobilized thereon, directly or indirectly, a reagent constructed so as to interact with another assay reagent during use so as to produce an optically measurable result, whereby during use said excitation radiation traverses said transduction region at least twice.

9. A waveguide sensor according to claim 8 useful for the optical assay of a ligand in a sample, wherein the reagent immobilized on the transduction region is either a specific binding partner for the ligand or a ligand analogue.

10. A waveguide sensor according to claim 9 wherein said waveguide further comprises an output grating structure constructed so a to couple signal radiation out of the waveguide, whereby said signal radiation has a wavelength different from said excitation radiation.

11. A waveguide sensor according to claim 10 wherein said waveguide further comprises a second reflecting grating structure constructed so as to reflect said signal radiation propagating within the waveguide.

12. A waveguide sensor according to claim 8 wherein said waveguide is substantially circular with a plurality of said input and reflecting grating structures and said transduction regions disposed concentrically.

13. In a method of assaying for a ligand in a sample which method comprises incubating the sample with components (a) a specific binding partner for the ligand and (b) a ligand analogue or a specific binding partner for the ligand, one of said components being immobilised on the surface of a transduction region of a planar waveguide and the other of said components carrying a fluorophore, said fluorophore becoming bound to said transduction region as a result of complex formation;

propagating excitation radiation in the waveguide so as to cause said bound fluorophore to emit signal radiation into the waveguide; and

measuring said signal radiation to determine the presence or amount of said ligand;

the improvement wherein said waveguide comprises an input grating structure constructed so as to couple excitation radiation into the waveguide so as to propagate therethrough, a reflecting grating structure spacially disposed from said input grating structure and constructed so as to reflect said excitation radiation propagating within the waveguide, and said transduction region being located between said input grating structure and said reflecting grating structure, whereby during said assay said excitation radiation traverses said transduction region at least twice.

14. The method of claim 13 wherein said waveguide additionally comprises an output grating structure constructed so as to couple signal radiation out of the waveguide.

15. The method of claim 14 wherein said waveguide additionally comprises a second reflecting grating structure constructed so as to reflect said signal radiation propagating within the waveguide.

16. In a method of assaying for a ligand in a sample which method comprises incubating the sample with components (a) a specific binding partner for the ligand and (b) a ligand analogue or a specific binding partner for the ligand, one of said components being immobilised on the surface of a transduction region of a planar waveguide, the immobilised component or the surface of the transduction region having a fluorophore bound thereto, and the other of said components being such that on complex formation the signal radiation emitted by said fluorophore is quenched;

propagating excitation radiation into the waveguide so as to cause said fluorophore to emit signal radiation into the waveguide; and

measuring the extent to which said signal radiation is quenched to determine the presence or amount of said ligand;

the improvement wherein said waveguide comprises an input grating structure constructed so as to couple excitation radiation into the waveguide so as to propagate therethrough, a reflecting grating structure spacially disposed from said input grating structure and constructed so as to reflect said excitation radiation propagating within the waveguide, and said transduction region being located between said input grating structure and said reflecting grating structure, whereby during said assay said excitation radiation traverses said transduction region at least twice.

17. The method of claim 16 wherein said waveguide additionally comprises an output grating structure constructed so as to couple signal radiation out of the waveguide.

18. The method of claim 17 wherein said waveguide additionally comprises a second reflecting grating structure constructed so as to reflect said signal radiation propagating within the waveguide.