Enzyme electrochemical sensor electrode and method of making it
First Claim
1. An electrochemical sensor electrode, comprising:
- an electronic conductor, anda thin coating on said conductor of a perfluorosulfonic acid polymer having an enzyme dispersed therein effective to catalyze a reaction between a selected substance and oxygen to generate hydrogen peroxide in said polymer for detection at said conductor.
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Accused Products
Abstract
An electrochemical sensor electrode is formed from an electronic conductor coated with a casting solution containing a perfluorosulfonic acid ionomer and a selected enzyme. The selected enzyme catalyzes a reaction between a predetermined substance in a solution and oxygen to form an electrochemically active compound that is detected at the electronic conductor. The resulting perfluorosulfonic acid polymer provides a stable matrix for the enzyme for long lived enzyme activity, wherein only thin coatings are required on the metal conductor. The polymer also advantageously repels interfering substances from contacting the enzyme and contains quantities of oxygen to maintain a sensing capability during conditions of oxygen depletion in the sample. In one particular embodiment, glucose oxidase is mixed with the perfluorosulfonic acid ionomer to form an electrode for glucose detection.
1067 Citations
9 Claims
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1. An electrochemical sensor electrode, comprising:
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an electronic conductor, and a thin coating on said conductor of a perfluorosulfonic acid polymer having an enzyme dispersed therein effective to catalyze a reaction between a selected substance and oxygen to generate hydrogen peroxide in said polymer for detection at said conductor. - View Dependent Claims (2, 3)
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4. An electrochemical sensor electrode, consisting essentially of:
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an electronic conductor, and a thin coating on said conductor of a perfluorosulfonic acid polymer having an enzyme dispersed therein effective to catalyze a reaction between a selected substance and oxygen to generate hydrogen peroxide in said polymer for detection at said conductor. - View Dependent Claims (5)
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6. A method of making an electrochemical sensor electrode using a selected enzyme to determine the concentration of a predetermined substance, comprising the steps of:
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providing an electronic conductor; providing a perfluorosulfonic acid ionomer in an alcohol solution; neutralizing said solution to form a pH which does not affect said enzyme; adding said enzyme to said ionomer solution in an amount effective to provide a desired sensitivity for said concentration of said predetermined substance; and applying said ionomer solution with said enzyme to said conductor. - View Dependent Claims (7, 8, 9)
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Specification