Glucose sensor with gel-immobilized glucose oxidase and gluconolactonase

• US 5,082,786 A
• Filed: 11/28/1988
• Issued: 01/21/1992
• Est. Priority Date: 11/26/1987
• Status: Expired due to Fees

• Current Assignee: NEC Corporation
• Original Assignee: NEC Corporation
• Inventors: Nakamoto, Shinya
• Primary Examiner(s): Lacey, David L.
• Assistant Examiner(s): Waack, Janelle D.
• Application Number:
  US07/276,663
• Time in Patent Office: 1,149 Days
• Field of Search: 435/288, 435/18, 435/25, 435/817, 435/175, 435/177, 435/14
• US Class Current: 257/253
• CPC Class Codes: C12M 21/18 Apparatus specially designed for the use of free, immobilized or carrier-bound enzymes
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12M 41/26 of pH
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A glucose sensor in which a gel is in contact with a pH sensing element. The gel contains immobilized glucose oxidase and gluconolactonase (EC 3.1.1.17). Gluconolactonase is present in an amount effective to accelerate the hydrolysis of D-glucono-δ-lactone.

Claims

1. A glucose sensor comprising (1) a gel in which glucose oxidase is immobilized and (2) a pH sensing element in sensing contact with said gel wherein said gel contains gluconolactonase (EC 3.1.1.17) in an amount effective to accelerate the hydrolysis of D-glucono-δ

-lactone.

2. A glucose sensor as set forth in claim 1 wherein the pH sensing element is a pH-ISFET.

3. A glucose sensor as set forth in claim 1 wherein the pH sensing element is a pH glass electrode.

4. A glucose sensor as set forth in claim 1 wherein the gel contains crosslinkable polymer.

5. A glucose sensor as set forth in claim 1 wherein said gluconolactonase amount in said gel is in an amount of more than 500 units per one cubic centimeter up to a limit in which concentration is still effective to accelerate the hydrolysis of D-glucono-δ

-lactone.