Centrifuge vessel for automated solid-phase immunoassay

• US 5,084,240 A
• Filed: 07/13/1990
• Issued: 01/28/1992
• Est. Priority Date: 07/25/1988
• Status: Expired due to Term

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: Cirrus Diagnostics, Inc. (Siemens AG)
• Inventors: Babson, Arthur L.
• Primary Examiner(s): WARDEN, JILL ALICE
• Application Number:
  US07/552,063
• Time in Patent Office: 564 Days
• Field of Search: 422/69, 422/72, 422/102, 494/43, 494/44, 436/524
• US Class Current: 422/72
• CPC Class Codes: G01N 33/5304 Reaction vessels, e.g. agglutination plates for solid-phase systems G01N33/543

Abstract

A centrifuge vessel (10) for performing automated immunoassays is disclosed. The centrifuge vessel (10) comprises a center tube (11) and an outer waste chamber (15). A biomaterial (18) is held within the center tube (11) and is capable of binding specific analytes in test samples. In operation, the centrifuge vessel (10) is rotated at high speed about its longitudinal axis, thereby causing all fluid within the center tube (11) to be transported into the outer waste chamber (15) while the analyte of interest remains bound to the biomaterial (18) positioned within the center tube (11).

Claims

1. A fluid transfer vessel designed for rotation at high speed about a central vertical axis for use in performing assays on liquid biological samples introduced into said fluid transfer vessel, comprising:

a tube having a conical interior wall tapering outwardly from said central vertical axis as said wall extends from a closed bottom end of said tube to an open top end of said tube, wherein centrifugal force generated as said fluid transfer vessel rotates at high speed about said central vertical axis urges said liquid biological sample to flow along said tapered wall toward said open top end and causes said liquid biological sample to spray from said open top end outwardly away from said central vertical axis;

a solid support having a biomaterial attached thereto for binding an analyte in said biological sample, said solid support positioned inside said tube but not attached to said tube;

means, secured to said tube, for catching and holding said liquid biological sample sprayed from said open top end of said tube when said fluid transfer vessel is at rest with respect to rotation about said central vertical axis, said means for catching and holding said liquid biological sample preventing said liquid bioligical sample sprayed from said open tube end of said tube from flowing back into said tube when said fluid transfer vessel is at rest; and

means for preventing said solid support from being transported from said tube into said means for catching and holding said liquid bioligical sample sprayed from said open top end of said tube.

2. A fluid transfer vessel as recited in claim 1 wherein said means for preventing said solid support from being transported from said tube into said mans for catching and holding said liquid biological sample sprayed from said open top end of said tube comprises a cap positioned a fixed distance from said open top end of said tube, said fixed distance from aid tube being smaller than a diameter of said solid support.

3. A fluid transfer vessel as recited in claim 2 wherein said cap is a puncturable material wherein said biological sample is introduced into said tube of said fluid transfer vessel through said puncturable material.

4. A fluid transfer vessel as recited in claim 3 wherein said puncturable material is foil.

5. A fluid transfer vessel as recited in claim 3 wherein said puncturable material is a plastic membrane.

6. A fluid transfer vessel as recited in claim 2 wherein said cap is a plastic material having an opening therethrough wherein said biological sample is introduced into said tube of said fluid transfer vessel through said opening.

7. A fluid transfer vessel as recited in claim 1 wherein said means for catching and holding said biological sample sprayed from said top end of said tube comprises a waste chamber connected to said tube having a side wall cooaxial with said tube and a closed bottom end.

8. A fluid transfer vessel as recited in claim 7 wherein said waste chamber is connected to aid tube on an outside wall of said tube at a point approximately half way between said closed bottom end and said open top end of said tube.

9. A fluid transfer vessel as recited in claim 7 wherein said means for preventing said solid support from being transported from said tube into said means for catching and holding said liquid biological sample sprayed from said open top end of said tube comprises a cap positioned a fixed distance from said open tube end of said tube, said fixed distance from said tube being smaller than a diameter of said solid support, said cap being secured to said side wall of said waste chamber.

10. A fluid transfer vessel as recited in claim 9 wherein said cap is secured to said side wall of said waste chamber by a weld.