Centrifuge vessel for automated solid-phase immunoassay
First Claim
1. A fluid transfer vessel designed for rotation at high speed about a central vertical axis for use in performing assays on liquid biological samples introduced into said fluid transfer vessel, comprising:
- a tube having a conical interior wall tapering outwardly from said central vertical axis as said wall extends from a closed bottom end of said tube to an open top end of said tube, wherein centrifugal force generated as said fluid transfer vessel rotates at high speed about said central vertical axis urges said liquid biological sample to flow along said tapered wall toward said open top end and causes said liquid biological sample to spray from said open top end outwardly away from said central vertical axis;
a solid support having a biomaterial attached thereto for binding an analyte in said biological sample, said solid support positioned inside said tube but not attached to said tube;
means, secured to said tube, for catching and holding said liquid biological sample sprayed from said open top end of said tube when said fluid transfer vessel is at rest with respect to rotation about said central vertical axis, said means for catching and holding said liquid biological sample preventing said liquid bioligical sample sprayed from said open tube end of said tube from flowing back into said tube when said fluid transfer vessel is at rest; and
means for preventing said solid support from being transported from said tube into said means for catching and holding said liquid bioligical sample sprayed from said open top end of said tube.
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0 Petitions
Accused Products
Abstract
A centrifuge vessel (10) for performing automated immunoassays is disclosed. The centrifuge vessel (10) comprises a center tube (11) and an outer waste chamber (15). A biomaterial (18) is held within the center tube (11) and is capable of binding specific analytes in test samples. In operation, the centrifuge vessel (10) is rotated at high speed about its longitudinal axis, thereby causing all fluid within the center tube (11) to be transported into the outer waste chamber (15) while the analyte of interest remains bound to the biomaterial (18) positioned within the center tube (11).
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Citations
10 Claims
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1. A fluid transfer vessel designed for rotation at high speed about a central vertical axis for use in performing assays on liquid biological samples introduced into said fluid transfer vessel, comprising:
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a tube having a conical interior wall tapering outwardly from said central vertical axis as said wall extends from a closed bottom end of said tube to an open top end of said tube, wherein centrifugal force generated as said fluid transfer vessel rotates at high speed about said central vertical axis urges said liquid biological sample to flow along said tapered wall toward said open top end and causes said liquid biological sample to spray from said open top end outwardly away from said central vertical axis; a solid support having a biomaterial attached thereto for binding an analyte in said biological sample, said solid support positioned inside said tube but not attached to said tube; means, secured to said tube, for catching and holding said liquid biological sample sprayed from said open top end of said tube when said fluid transfer vessel is at rest with respect to rotation about said central vertical axis, said means for catching and holding said liquid biological sample preventing said liquid bioligical sample sprayed from said open tube end of said tube from flowing back into said tube when said fluid transfer vessel is at rest; and means for preventing said solid support from being transported from said tube into said means for catching and holding said liquid bioligical sample sprayed from said open top end of said tube. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification