Process for producing alcohol using yeast transformed by Rhizopus glucoamylase gene

• US 5,084,385 A
• Filed: 04/14/1989
• Issued: 01/28/1992
• Est. Priority Date: 12/15/1984
• Status: Expired due to Fees

• Current Assignee: Suntory Ltd (Kotobuki Realty Co., Ltd.)
• Original Assignee: Suntory Ltd (Kotobuki Realty Co., Ltd.)
• Inventors: Nakamura, Norihisa, Yoshizumi, Hajime, Tanaka, Yoshikazu, Ashikari, Toshihiko, Shibano, Yuji
• Primary Examiner(s): Stone, Jacqueline
• Assistant Examiner(s): Hendricks, Keith D.
• Application Number:
  US07/338,395
• Time in Patent Office: 1,019 Days
• Field of Search: 435/162, 435/161, 435/172.3, 435/205, 435/939, 435/96, 935/10, 935/28
• US Class Current: 435/96
• CPC Class Codes: C12N 9/2428 Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
• CPC Class Codes: C12P 7/06 Ethanol, i.e. non-beverage
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y10S 435/939 Rhizopus

Abstract

This invention provides a process for producing ethanol from a starchy material, wherein the starchy material is simultaneously subjected to saccharification and subsequent fermentation by use of a yeast host which has been transformed by an expressible recombinant vector comprising a glucoamylase gene derived from a fungus of the genus Rhizopus.

Claims

1. A process for producing ethanol from a starchy material, wherein the starchy material is simultaneously subjected to saccharification and subsequent fermentation by use of a yeast host which has been transformed by an expressible recombinant vector comprising a glucoamylaser gene encoding a polypeptide comprising the amino acid sequence (III):

##STR1##

2. A process according to claim 1, wherein the glucoamylase gene is derived from fungus of Rhizopus oryzae.

3. A process according to claim 1, wherein the vector is selected from the group consisting of plasmid pCGA449, pCGA469, pYGA22149, PYGA2169 and pYGA195.

4. A process according to claim 1, wherein the host is Saccharomyces cerevisiae.

5. A process according to claim 1, wherein the glucoamylaser gene comprises nucleotide sequence (IV):

##STR2##

6. A process according to claim 1, wherein the glucoamylaser gene is linked with a DNA sequence capable of effecting expression of the gene encoding said polypeptide.