Column separation system for electrophoresis with sample pretreatment
First Claim
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1. An apparatus, useful for separating molecules by capillary electrophoresis, comprising:
- an elongated tube having a substantially cylindrical bore, said elongated tube defining a first portion and a second portion, wherein the first portion includes means for filtering molecules on the basis of molecular weight, the second portion of a construction sufficient to permit separations of molecules by electrical charge when flowed through the bore, the first and second portions in sequential fluid communication.
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Abstract
Capillary tubes useful for performing capillary zone electrophoresis separation techniques are modified to provide sample pre-treatment in situ. In one preferred embodiment, a polyacrylamide stacking gel is formed in a capillary tube and separated from the free zone of the tube by a polystyrene frit. The modified capillary permits effective filtration of macromolecules from analysis or their retardation to allow for sequential analysis.
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Citations
7 Claims
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1. An apparatus, useful for separating molecules by capillary electrophoresis, comprising:
an elongated tube having a substantially cylindrical bore, said elongated tube defining a first portion and a second portion, wherein the first portion includes means for filtering molecules on the basis of molecular weight, the second portion of a construction sufficient to permit separations of molecules by electrical charge when flowed through the bore, the first and second portions in sequential fluid communication. - View Dependent Claims (2, 3, 4, 5, 6)
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7. The apparatus as in clam 1 wherein the filtering means of the first portion includes a selectively permeable polymer substantially plugging an end of the bore adjacent to the first portion.
Specification