Multilayered assay element

• US 5,087,421 A
• Filed: 05/19/1989
• Issued: 02/11/1992
• Est. Priority Date: 05/20/1988
• Status: Expired due to Fees

• Current Assignee: Konica Corporation (Konica Minolta Inc.)
• Original Assignee: Konica Corporation (Konica Minolta Inc.)
• Inventors: Okaniwa, Kenichiro, Akashi, Tomoji, Koyama, Mikio, Kawakatsu, Satoshi
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): ALEXANDER, LYLE
• Application Number:
  US07/353,942
• Time in Patent Office: 998 Days
• Field of Search: 422/56-58, 430/216, 430/213, 8/555, 8/551, 436/166
• US Class Current: 422/423
• CPC Class Codes: G01N 33/523 the element being adapted for a specific analyte
• CPC Class Codes: G01N 33/728 Bilirubin; including biliverdin

Abstract

Disclosed is a multilayered assay element in which the influence by the bilirubin contained in the sample fluid is substantially eliminated. The assay element of the present invention contains a bilirubin-trapping agent of the formula

(A).sub.x (B).sub.y (C).sub.z                              [I]

(wherein A represents a monomer unit having a copolymerizable unsaturated double bond and a quaternary ammonium salt, B represents a monomer unit having a copolymerizable unsaturated double bond, C represents a monomer unit having at least two copolymerizable unsaturated double bonds and x, y and z represents the number mol % of 100-10, 90-0 and 10-0, respectively) which bilirubin-trapping agent is incorporated in a region which is upper than the reagent layer.

Claims

1. A multilayered assay element comprising:

a support ;

a reagent layer formed on the support; and

,

a spreading layer formed on the reagent layer, said spreading layer having incorporated therein a bilirubin-trapping agent of the formula I

space="preserve" listing-type="equation">(A)(B)(C) I

wherein

A represents a monomer unit represented by one of the formulae II to VII ##STR13## wherein R₁ represents a hydrogen atom or methyl group, R₂, R₃ and R₄, the same or different represent an alkyl, aralkyl, cycloalkyl or cycloaralkyl group, and X^- represents a monovalent anion, ##STR14## wherein R₁, R₂, R₃, R₄ and X^- have the same meaning as in the formula II, ##STR15## wherein R₁, R₂, R₃, R₄ and X^- have the same meaning as in the formula II, and n represents an integer of 1 to 4, ##STR16## wherein R¹, R₂, R₃, and R₄ and X^- have the same meaning as in the formula II, ##STR17## wherein R₁, R₂, R₃, R₄ and X^- have the same meaning as in the formula II, and m represents an integer of 1 to 4, ##STR18## wherein R₁, R₂, R₃, R₄ and X^- have the same meaning as in the formula II, and m has the same meaning as in the formula VI;

B represents a monomer unit having a copolymerizable unsaturated double bond;

C represents a monomer unit having at least two copolymerizable unsaturated double bonds; and

x, y and z represent the number mol% of 100-10, 90-0 and 10-0, respectively.

2. The element of claim 1, wherein the bilirubin-trapping agent is in the form of latex.

3. The element of claim 1, wherein the spreading layer comprises a fibrous material bounded with a binder, and the bilirubin-trapping agent is contained in the spreading layer in the amount of not less than 10% by weight of the binder.

4. The element of claim 3, wherein the ratio of the fibrous material to the binder is 98:

2 to 80;

20 based on weight.