Electrochemical biosensor based on immobilized enzymes and redox polymers
First Claim
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1. An enzyme electrode for sensing the presence of at least one component of a mixture of components, said enzyme electrode comprising:
- (a) an enzyme, the catalytic activity of said enzyme being indicative of said component,(b) a non-physiological electron transfer mediator between the enzyme and electron collector, said mediator being covalently attached to an insoluble, flexible siloxane polymer backbone in sufficient number to form an electron relay system,(c) and an electron collector,said enzyme and said mediator-containing siloxane polymer backbone being in an immobilized state in contact with said electron collector.
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Abstract
The present invention relates to an electrochemical enzyme biosensor for use in liquid mixtures of components for detecting the presence of, or measuring the amount of, one or more select components. The enzyme electrode of the present invention is comprised of an enzyme, an artificial redox compound covalently bound to a flexible polymer backbone and an electron collector.
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Citations
10 Claims
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1. An enzyme electrode for sensing the presence of at least one component of a mixture of components, said enzyme electrode comprising:
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(a) an enzyme, the catalytic activity of said enzyme being indicative of said component, (b) a non-physiological electron transfer mediator between the enzyme and electron collector, said mediator being covalently attached to an insoluble, flexible siloxane polymer backbone in sufficient number to form an electron relay system, (c) and an electron collector, said enzyme and said mediator-containing siloxane polymer backbone being in an immobilized state in contact with said electron collector. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification