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Method for impregnating tissue samples in paraffin

  • US 5,089,288 A
  • Filed: 06/01/1990
  • Issued: 02/18/1992
  • Est. Priority Date: 06/24/1989
  • Status: Expired due to Fees
First Claim
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1. In a method for impregnating a tissue sample with paraffin, the improvement comprising:

  • fixing the tissue sample with a fixing agent;

    dewatering the tissue sample by using alcohol;

    treating the tissue sample in isopropyl-alcohol; and

    soaking the tissue sample in liquid paraffin by the tissue sample being placed into the paraffin in a working chamber at a pregiven operating temperature, the operating temperature being in the range of about 52°

    to 60°

    C. in order to keep the paraffin in a liquid state and therefore lower than the boiling temperature of isopropyl alcohol at a normal atmospheric pressure;

    the tissue sample being soaked in a closed, evacuated working chamber under the influence of ultrasound on the tissue sample and on the liquid paraffin enclosing the tissue sample; and

    the vacuum in the working chamber being chosen such that the boiling point of the isopropyl alcohol equals the operating temperature at the pressure existing in the working chamber.

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