Method for impregnating tissue samples in paraffin
First Claim
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1. In a method for impregnating a tissue sample with paraffin, the improvement comprising:
- fixing the tissue sample with a fixing agent;
dewatering the tissue sample by using alcohol;
treating the tissue sample in isopropyl-alcohol; and
soaking the tissue sample in liquid paraffin by the tissue sample being placed into the paraffin in a working chamber at a pregiven operating temperature, the operating temperature being in the range of about 52°
to 60°
C. in order to keep the paraffin in a liquid state and therefore lower than the boiling temperature of isopropyl alcohol at a normal atmospheric pressure;
the tissue sample being soaked in a closed, evacuated working chamber under the influence of ultrasound on the tissue sample and on the liquid paraffin enclosing the tissue sample; and
the vacuum in the working chamber being chosen such that the boiling point of the isopropyl alcohol equals the operating temperature at the pressure existing in the working chamber.
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Abstract
A method of impregnating a tissue sample with paraffin in which, in a treatment vessel, a tissue sample, which has been fixed with isopropyl alcohol, is maintained under vacuum and simultaneously the molten paraffin and tissue sample are subjected to ultrasonic vibration effective to remove the isopropyl alcohol from the tissue sample and to impregnate the tissue sample with the paraffin.
52 Citations
11 Claims
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1. In a method for impregnating a tissue sample with paraffin, the improvement comprising:
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fixing the tissue sample with a fixing agent; dewatering the tissue sample by using alcohol; treating the tissue sample in isopropyl-alcohol; and soaking the tissue sample in liquid paraffin by the tissue sample being placed into the paraffin in a working chamber at a pregiven operating temperature, the operating temperature being in the range of about 52°
to 60°
C. in order to keep the paraffin in a liquid state and therefore lower than the boiling temperature of isopropyl alcohol at a normal atmospheric pressure;the tissue sample being soaked in a closed, evacuated working chamber under the influence of ultrasound on the tissue sample and on the liquid paraffin enclosing the tissue sample; and
the vacuum in the working chamber being chosen such that the boiling point of the isopropyl alcohol equals the operating temperature at the pressure existing in the working chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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Specification