DNA probe diffraction assay and reagents
First Claim
1. A biograting for use in a light diffraction assay comprising a smooth surface having on said surface, a biological diffraction grating design of lines of active hybridizing reagent.
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Accused Products
Abstract
The assay of the subject invention uses DNA sequences as probes in a nucleic acid hybridization diffraction assay, to detect specific DNA sequences in a sample. Diffraction assay methodologies are applied to determine the presence and amount of analyte.
This invention involves a discovery in the areas of supporting surfaces for a biogrid or biograting which provide greatly reduced non-specific hybridization and binding. A preferred process of this invention involves manufacturing a biograting for use in a light diffraction assay, and comprises adhering a uniform layer of hybridizing reagent comprising a nucleotide sequence on a smooth, solid surface and exposing the surface to UV radiation through a shadow mask with a diffraction grating pattern of lines to selectively deactivate the hybridizing reagent, leaving a biological diffraction grating design of lines of active hybridizing reagent. The smooth, solid surface is preferably selected from the group consisting of polysilicon and single crystalline silicon surfaces.
The diffraction hybridizing assay method of this invention for determining the presence or quantity of an analyte in an aqueous sample comprises contacting a nucleic acid sequence diffraction biogrid with the sample under proper circumstances and for a sufficient time to permit nucleic acid hybridization between a nucleic acid sequence probe and an analyte; separating the biogrid from the sample; illuminating the biogrid with light from a light source; and determining the light diffracted by the diffraction hybridization assay surface.
170 Citations
20 Claims
- 1. A biograting for use in a light diffraction assay comprising a smooth surface having on said surface, a biological diffraction grating design of lines of active hybridizing reagent.
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8. A process for manufacturing a biograting for use in a light diffraction assay, comprising:
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(a) adhering a uniform layer of hybridizing reagent on a smooth, solid surface; and (b) exposing the surface to UV radiation through a shadow mask with diffraction grating lines to selectively deactivate the hybridizing reagent to leave a biological diffraction grating design of lines of active hybridizing reagent. - View Dependent Claims (9, 10, 11, 12, 13, 14)
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15. An assay method for determining the presence or quantity of an analyte nucleotide sequence in an aqueous sample comprising
(a) contacting an assay surface with the sample for a time sufficient to permit binding of hybridizing reagent and analyte, the assay surface having on said surface, a light disturbing design of substantially non-light disturbing hybridizing reagent thereon, the hybridizing reagent being selected to hybridize selectively with the analyte; - and
(b) illuminating the assay surface with light from a light source, and determining the light diffracted by the assay surface. - View Dependent Claims (16, 17, 18, 19, 20)
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Specification