Nucleotide compositions with linking groups

• US 5,091,519 A
• Filed: 05/01/1986
• Issued: 02/25/1992
• Est. Priority Date: 05/01/1986
• Status: Expired due to Fees

• Current Assignee: Amoco Corporation (BP PLC)
• Original Assignee: Amoco Corporation (BP PLC)
• Inventors: Cruickshank, Kenneth
• Primary Examiner(s): Rollins, John W.
• Assistant Examiner(s): Wilson, James O.
• Application Number:
  US06/858,600
• Time in Patent Office: 2,126 Days
• Field of Search: 536/23, 536/29
• US Class Current: 536/26.23
• CPC Class Codes: C07D 405/04 directly linked by a ring-member-to-ring-member bond
• CPC Class Codes: C07D 473/00 Heterocyclic compounds containing purine ring systems
• CPC Class Codes: C07H 19/06 Pyrimidine radicals
• CPC Class Codes: C07H 19/10 with the saccharide radical esterified by phosphoric or polyphosphoric acids

Abstract

The present invention includes nucleotide compositions having linking groups and the use and manner of making the nucleotide compositions. The nucleotide compositions are used to attach nonisotopic label moieties to nucleic acid probes.

Claims

1. A nucleotide having a linking group carried on an exocylic functional group of a nucleotide represented by the formula:

##STR1## wherein X is hydrogen, mono, di, or triphoshate, or dimethoxytrityl;

Z is hydrogen, hydroxyl, acetyl or a phosphoramidite, and A is represented by the formula;

space="preserve" listing-type="equation">--(CH.sub.2).sub.w NHCOCK.sub.3

wherein K is selected from the group of halogens consistently of Cl, Br, F, I and At and w is an integer from 3 to 9; and

B is the same as A or hydrogen or A and B, taken together with the exocylic nitrogen, are ##STR2##

2. The compound of claim 1 wherein Z is (methyl-N, N-diisopropyl) phosphoramidite.

3. A nucleotide having a linking group carried on an exocyclic functional group of a nucleotide, represented by the formula:

##STR3## wherein X is hydrogen, mono, di, or triphosphate, or dimethoxytrityl;

Z is a phosphoramidite;

A is selected from the group consisting of the following;

space="preserve" listing-type="equation">--(CH.sub.2).sub.m NH.sub.2

wherein m is an integer from 1 to 9 and;

B is the same as A or hydrogen.

4. The compound of claim 3 wherein said phosphoramidite is (methyl-N, N-diisopropyl) phosphoramidite.