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Laser excited confocal microscope fluorescence scanner and method

  • US 5,091,652 A
  • Filed: 06/01/1990
  • Issued: 02/25/1992
  • Est. Priority Date: 01/12/1990
  • Status: Expired due to Term
First Claim
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1. An improved gel scanner comprisingmeans for supporting a gel to be scanned,means for forming a light beam of predetermined wavelength;

  • a dichroic beam splitter for receiving and directing said beam toward a supported gel to be scanned;

    an objective lens for receiving said beam and focusing the beam in a selected volume of gel to cause fluorescence emission of light at a different wavelength and collecting the emitted light from samples in said selected volume and directing the emitted light to said dichroic beam splitter which passes said emitted light of different wavelengths and reflects light at said predetermined wavelength;

    a spatial filter for receiving and passing the emitted light from said volume while rejecting background and scattered light;

    means for providing relative movement between said focused beam and the gel being scanned,means for detecting said passed, emitted light and providing output signals, andmeans for processing said signals to provide an image of the fluorescence from the gel.

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