Nucleic acid probes
First Claim
Patent Images
1. A modified nucleotide having the structure ##STR22## wherein:
- R1 is a protected or unprotected --NH2, --COOH or --SH group;
R2 is a covalent bond or an amide linking moiety with the priviso that the amide linkage is oriented --NH--C(═
O)--;
R3 is hydrogen, methyl, bromine, fluorine or iodine;
R4 is selected from the group consisting of hydrogen, acid-sensitive, base-stable blocking groups and acyl capping groups;
R5 is a phosphorus derivative selected from the group consisting of PO3 H2, phosphotriesters, phosphodiesters, phosphites, phosphoramidites, H-phosphonates and phosphorothioates;
R6 is H, OH, or OR where R is an acid-sensitive, base-stable protecting group; and
x is an integer in the range of 1 and 8 inclusive,wherein the nucleotide may bound through R5 to a solid support.
3 Assignments
0 Petitions
Accused Products
Abstract
Modified nucleotides are provided which have the structure ##STR1## wherein R1 is a reactive group derivatizable with a detectable label, R2 is an optional linking moiety including an amide, thioether or disulfide linkage or a combination thereof, R3 is hydrogen, methyl, bromine, fluorine or iodine, R4 is hydrogen, an acid-sensitive, base-stable blocking group of an acyl capping group, R5 is hydrogen or a phosphorus derivative, R6 is H, OH, or OR where R is a protecting group and x is an integer in the range of 1 and 8 inclusive. Methods of synthesizing the derivatizable nucleotide are disclosed, as are labeled polynucleotide probes prepared therefrom.
219 Citations
14 Claims
-
1. A modified nucleotide having the structure ##STR22## wherein:
- R1 is a protected or unprotected --NH2, --COOH or --SH group;
R2 is a covalent bond or an amide linking moiety with the priviso that the amide linkage is oriented --NH--C(═
O)--;R3 is hydrogen, methyl, bromine, fluorine or iodine; R4 is selected from the group consisting of hydrogen, acid-sensitive, base-stable blocking groups and acyl capping groups; R5 is a phosphorus derivative selected from the group consisting of PO3 H2, phosphotriesters, phosphodiesters, phosphites, phosphoramidites, H-phosphonates and phosphorothioates; R6 is H, OH, or OR where R is an acid-sensitive, base-stable protecting group; and x is an integer in the range of 1 and 8 inclusive, wherein the nucleotide may bound through R5 to a solid support. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
- R1 is a protected or unprotected --NH2, --COOH or --SH group;
Specification