Method and material for measurement of oxygen concentration
First Claim
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1. An analyte sensing system which comprises:
- a species which displays an output when exposed to external excitation, said output quenched by the presence of an analyte, said species exhibiting a level of said quenching in output varying with the frequency of output, said species being a polynuclear aromatic hydrocarbon based fluorescent dye selected from the group consisting of perylene derivatives;
an analyte permeable silicone matrix in which said species is dispersed or immobilized, said matrix being adapted to be exposed directly or indirectly to a sample containing an analyte to be measured;
a means to excite said species at a first frequency;
a means to measure the output of said species at a plurality of frequencies; and
a means to ratio said output in said plurality of frequencies to thereby measure the level of analyte in said sample.
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Abstract
The analyte sensing system utilizes a sensor indicator which may be a perlyene derivative, coronene, or naphtho [8,1,2-abc] coronene in an analyte permeable silicone matrix. The matrix is irradiated with light of a specific wavelength, and fluorescence emissions of the dye indicator is measured over two different emission spectra having different sensitivities to the analyte. The emission spectrum which is less sensitive to quenching by the analyte can be used as a reference for determining the analyte concentration based upon a ratio of the intensities of the different emission spectra.
31 Citations
12 Claims
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1. An analyte sensing system which comprises:
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a species which displays an output when exposed to external excitation, said output quenched by the presence of an analyte, said species exhibiting a level of said quenching in output varying with the frequency of output, said species being a polynuclear aromatic hydrocarbon based fluorescent dye selected from the group consisting of perylene derivatives; an analyte permeable silicone matrix in which said species is dispersed or immobilized, said matrix being adapted to be exposed directly or indirectly to a sample containing an analyte to be measured; a means to excite said species at a first frequency; a means to measure the output of said species at a plurality of frequencies; and a means to ratio said output in said plurality of frequencies to thereby measure the level of analyte in said sample. - View Dependent Claims (2, 3, 4)
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5. A method of sensing the concentration of an analyte in a fluid medium which comprises:
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exposing a sample of said fluid medium to an indicator which displays a variation in output as a function of frequency when exposed to an analyte, said indicator being a polynuclear aromatic hydrocarbon based fluorescent dye selected from the group consisting of perylene derivatives and said indicator being dispersed or immobilized in an analyte permeable silicone matrix; activating said indicator with radiation; measuring the output of said indicator at a plurality of frequencies; and determining the concentration of said analyte on the basis of the ratio of said plurality of outputs of said indicator. - View Dependent Claims (6, 7, 8)
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9. An analyte sensing apparatus which comprises:
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a species of polynuclear aromatic hydrocarbon based fluorescent dye selected from the group consisting of perylene derivatives, which produces an output when exposed to external excitation, said output varying as a function of the concentration of an analyte exposed to said species, said species further including a variation in output as a function of the frequency of said output when said species is exposed to a given concentration of said analyte; means to constrain a quantity of said species, said constraining means including an analyte permeable silicone matrix and further providing means to expose at least a portion of said species to said analyte; means to excite said species; means to measure the output of said species at a plurality of frequencies of said output; and means to derive the concentration of said analyte from said measurements. - View Dependent Claims (10, 11, 12)
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Specification