×

Nucleic acid fractionation by counter-migration capillary electrophoresis

  • US 5,096,554 A
  • Filed: 08/07/1989
  • Issued: 03/17/1992
  • Est. Priority Date: 08/07/1989
  • Status: Expired due to Term
First Claim
Patent Images

1. A method of fractionating a mixture of different molecular weight nucleic acid fragments, comprisingloading a liquid sample containing the nucleic acid fragments into one end of a microcapillary tube filled with a fluid electrolyte solution, where the inner wall surface of the tube contains negatively charged groups, one end of the tube is placed in fluid communication with an anodic reservoir containing a polymer solution of an uncharged polymer having a molecular weight of at least about 50,000 daltons, and the other tube end is placed in communication with an cathodic reservoir, andapplying a voltage between the anodic and cathodic reservoirs which is effective to draw said polymer solution into and through the tube by electroosmotic flow, at a fluid flow rate in the tube which is greater, in the direction of the cathodic reservoir, than the molecular-weight dependent rates of migration of such nucleic acid fragments, in the direction of the anodic reservoir, such larger molecular weight nucleic acid fragments move more rapidly toward the cathodic reservoir.

View all claims
  • 10 Assignments
Timeline View
Assignment View
    ×
    ×