Crystal growing apparatus

• US 5,096,676 A
• Filed: 08/02/1990
• Issued: 03/17/1992
• Est. Priority Date: 01/27/1989
• Status: Expired due to Fees

• Current Assignee: Alexander Mcpherson, Daniel Morris
• Original Assignee: Alexander Mcpherson, Daniel Morris
• Inventors: Morris, Daniel, McPherson, Alexander
• Primary Examiner(s): Straub, Gary P.
• Application Number:
  US07/563,670
• Time in Patent Office: 593 Days
• Field of Search: 156/62, 156/607, 156/608, 156/DIG. 113, 206/521.1, 206/521.2, 206/521.9, 206/562, 206/568, 206/521.6, 422/109, 422/104, 422/245
• US Class Current: 117/206
• CPC Class Codes: C30B 29/58 Macromolecular compounds
• CPC Class Codes: C30B 7/00 Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions from molten solvents C30B9/00; by normal or gradient freezing C30B11/00; under a protective fluid C30B27/00
• CPC Class Codes: Y10T 117/1024 for crystallization from liquid or supercritical state

Abstract

An apparatus for growing diffraction quality, protein crystals by vapor diffusion techniques. The apparatus includes a plurality of equilibrating solution reservoirs each having a side wall terminating in a top wall disposed in a first plane; a column disposed within each of the equilibrating solution reservoirs, each column having a protein solution receptacle including a side wall terminating in a top wall disposed in a second plane spaced from the first plane; and a cover having a non-greasy adhesive on one surface therof for sealable engagement with the top walls of the equilibrating solution reservoirs for sealing the reservoirs relative to atmosphere.

Claims

1. An apparatus for growing protein crystals comprising:

(a) a plurality of equilibrating solution reservoirs each having a bottom wall and an inclosing side wall so fixed in said apparatus as to terminate in a top disposed in a first plane;

(b) a drop supporting means for supporting a protein drop disposed within each of said equilibrating solution reservoirs, each said drop supporting means comprising an upstanding column spaced apart from said side wall and having a protein solution depression disposed on the top thereof, the top of said protein solution depression being located below and parallel said first plane; and

(c) cover means for engagement with said top of said equilibrating solution reservoirs for sealing said reservoirs wherein each of said protein solution depressions is in communication only with said equilibrating reservoir within which it is disposed.

2. An apparatus as defined in claim 1 in which said protein solution receptacle is generally cup shaped and formed from a plastic material.

3. An apparatus as defined in claim 1 in which said cover means comprises a thin sheet of transparent material provided with an adhesive for sealable engagement with said top of each of said equilibrating solution reservoirs.

4. An apparatus as defined in claim 1 in which each of said equilibrating solution reservoirs is circular in cross-section.

5. An apparatus as defined in claim 4 in which each of said columns is generally circular in cross-section.

6. An apparatus as defined in claim 5 in which said bottom wall of each of said equilibrating solution reservoirs is formed with said column by molding.

7. An apparatus for growing protein crystals, comprising:

(a) interconnected side and end walls defining an enclosure, said walls terminating in a top edge disposed within a first plane;

(b) a plurality of equilibrating solution reservoirs formed interiorly of said enclosure;

each said reservoir including a bottom wall and an upstanding inclosing side wall terminating in an edge disposed within said first plane;

(c) an upstanding column disposed within each of said equilibrating solution reservoirs, each said column having a generally cup shaped protein solution carrying receptacle having a wall terminating in an edge disposed in a second plane, said second plane being spaced below said first plane and parallel to said first plane; and

,

(d) a sealable cover for engagement with said edges of said side and end walls and for engagement with said edges of each of said reservoirs wherein each said protein solution carrying receptacle is in communication only with said equilibrating reservoir within which it is disposed.

8. An apparatus as defined in claim 7 in which each said column is integrally formed with said bottom wall of said reservoir within which said column is disposed.

9. An apparatus as defined in claim 7 in which said side walls, said end walls, said equilibrating solution reservoirs and said columns are integrally formed from a polystyrene.

10. An apparatus as defined in claim 7 in which each of said side and edge walls includes an expansion away from respective side and edge walls, the tops of each said expansion disposed a third plane spaced below said first plane so more than one of said apparatus can be placed in stackable arrangement with the bottom edges of said expansion of the upper apparatus of a stack being in surrounding contact with the top of said side and end walls of the next lower apparatus.

11. An apparatus as defined in claim 7 further including porous means disposed within each said equilibrating solution reservoir for absorbing the equilibrating solution.

12. An apparatus as defined in claim 11 in which said porous means comprises annular shaped members constructed from a porous plastic foam.