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Whole blood assays using porous membrane support devices

  • US 5,096,809 A
  • Filed: 07/25/1988
  • Issued: 03/17/1992
  • Est. Priority Date: 07/25/1988
  • Status: Expired
First Claim
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1. A method for analyzing whole blood for an analyte recognized by an analyte specific reagent, comprising the steps of:

  • applying whole blood to a liquid permeable support layer of an assay device, wherein said layer has said analyte specific reagent fixed thereto prior to said introduction of said blood, and wherein said whole blood is applied to said layer;

    drawing colored components of said blood through said layer; and

    forming a color on said layer in response to interaction between said analyte and said analyte specific reagent when analyte is present in said blood, thereby visually indicating on said layer the presence or absence of said analyte in said blood.

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