Plant promoters

• US 5,097,025 A
• Filed: 12/01/1989
• Issued: 03/17/1992
• Est. Priority Date: 08/01/1989
• Status: Expired due to Term

• Current Assignee: Rockefeller University
• Original Assignee: Rockefeller University
• Inventors: Benfey, Philip N., Chua, Nam-Hai
• Primary Examiner(s): Weimar, Elizabeth C.
• Assistant Examiner(s): Benzion, Gary
• Application Number:
  US07/444,591
• Time in Patent Office: 837 Days
• Field of Search: 435/320, 435/91, 435/172.1, 435/172.3, 435/320.1, 935/6, 935/25, 935/33, 935/30, 935/41, 536/27
• US Class Current: 536/23.6
• CPC Class Codes: C12N 15/8216 Methods for controlling, regulating or enhancing expression of transgenes in plant cells
• CPC Class Codes: C12N 15/8223 Vegetative tissue-specific promoters
• CPC Class Codes: C12N 15/8234 Seed-specific, e.g. embryo, endosperm

Abstract

Five subdomains of the CaMV35S promoter are provided that cause tissue specific and/or developmentally regulated expression of chimeric genes in plants. These subdomains act as promoters for use in transformed plant cells, seeds and transgenic plants. Some of the subdomains require fusion to domain A for expression. Subdomains B2, B3, B4, and B5 exhibit expression when fused to the minimal promoter sequence in mature plants whereas only B2 and B3 confer expression in seeds and only B2, B3 and B4 confer expression at the seedling stage of development. The combination of subdomains B4 and B5 confers expression at all stages of development as does the B1+TGACG motif combination when each combination is fused with the minimal promoter sequence. The nucleotide sequence and DNA molecule that function as the enhancers are provided.

Claims

1. An isolated DNA segment consisting of the nucleotide sequence:

5'"'"'-CGACCAGCAT CGTGGAAAAA GAAGACGTTC CAACCACGTC TTCAAAGC-3'"'"'.

2. A DNA sequence consisting of a first nucleotide sequence:

5'"'"'-CGAGGAGCAT CGTGGAAAAA GAAGACGTTC CAACCACGTC TTCAAAGC-3'"'"' and

a second nucleotide sequence corresponding to domain A of the CaMV35S promoter coupled to said first nucleotide sequence by means of a synthetic multilinker.

3. A DNA sequence consisting of a first nucleotide sequence:

##STR1## and a second nucleotide sequence corresponding to domain A of the CaMV35S promoter coupled to said first nucleotide sequence by means of a synthetic multilinker.

4. A DNA sequence consisting of a first nucleotide sequence:

##STR2## and a second nucleotide sequence corresponding to domain A of the CaMV35S promoter coupled to said first nucleotide sequence by means of a synthetic multilinker.

5. A DNA sequence consisting of a first nucleotide sequence:

5'"'"'-TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATT-3'"'"' and

a second nucleotide sequence corresponding to domain A of the CaMV35S promoter coupled to said first nucleotide sequence by means of a synthetic multilinker.

6. An isolated DNA segment consisting of the nucleotide sequence:

##STR3##

7. An isolated DNA segment consisting of the nucleotide sequence:

##STR4##

8. An isolated DNA segment consisting of the nucleotide sequence:

5'"'"'-TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATT-3'"'"'.

9. A DNA sequence consisting of a first nucleotide sequence

5'"'"'-CGAGGAGCAT CGTGGAAAAA GAAGACGTTC CAACCACGTC TTCAAAGC-3'"'"' and

a second nucleotide sequence corresponding to the minimal promoter region of the CaMV35S promoter coupled to said first nucleotide sequence by means of a synthetic multilinker.

10. A DNA sequence consisting of a first nucleotide sequence ##STR5## and a second nucleotide sequence corresponding to the minimal promoter region of the CaMV35S promoter coupled to said first nucleotide sequence by means of a synthetic multilinker.

11. A DNA sequence consisting of a first nucleotide sequence ##STR6## and a second nucleotide sequence corresponding to the minimal promoter region of the CaMV35S promoter coupled to said first nucleotide sequence by means of a synthetic multilinker.

12. A DNA sequence consisting of a first nucleotide sequence

5'"'"'-TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATT-3'"'"' and

a second nucleotide sequence corresponding to the minimal promoter region of the CaMV35S promoter coupled to said first nucleotide sequence by means of a synthetic multilinker.