Process for the production of thyroglobulin
First Claim
1. In a process for the production of thyroglobulin having a thyroxine-to-triiodothyronine ratio (T4 /T3 ratio) comprising saline digestion mammalian thyroid glands to release thyroglobulin, precipitation of thyroglobulin by pH adjustment, denaturation of thyroglobulin by heating, and defatting thyroglobulin by extraction with alcohol, the improvement comprising:
- correlating the precipitating, denaturing, and defatting steps whereby the duration of the precipitating step is selected to produce thyroglobulin having a first T4 /T3 ratio substantially greater than 2.6 and the durations of the denaturing and defatting steps are selected in correlation with said precipitating step duration to lower said T4 /T3 value greater than 2.6 to a final value in the range of 2.6-3.4 and absolute levels of not less than about 0.7 μ
g/mg liothyronine and not less than about 2.1 μ
g/mg levothyroxine.
3 Assignments
0 Petitions
Accused Products
Abstract
The invention relates to a process for the production of thyroglobulin, which is used in the treatment of hypothyroidism, from hog thyroid glands. Ground hog thyroid glands are subjected to saline digestion to produce a thyroglobulin extract. The extract is precipitated by pH adjustment and then heated to a denaturing temperature. The denatured precipitated thyroglobulin solution is subjected to a separation step to produce crude wet thyroglobulin solids. The crude thyroglobulin solids are defatted and dewatered by solvent extraction. The resultant defatted thyroglobulin product can then be further worked up, for example, by drying, milling, and screening, to produce a powdered product. In order to achieve a final product having a T4 /T3 ratio of 2.6-3.4 and upon proteolysis a minimum of 0.7 μg/mg of levothyronine and 2.1 μg/mg of liothyronine, the time periods for the precipitating, denaturing, and/or defatting steps are carefully controlled so that the T4 /T3 ratio is within this desired range.
-
Citations
18 Claims
-
1. In a process for the production of thyroglobulin having a thyroxine-to-triiodothyronine ratio (T4 /T3 ratio) comprising saline digestion mammalian thyroid glands to release thyroglobulin, precipitation of thyroglobulin by pH adjustment, denaturation of thyroglobulin by heating, and defatting thyroglobulin by extraction with alcohol, the improvement comprising:
correlating the precipitating, denaturing, and defatting steps whereby the duration of the precipitating step is selected to produce thyroglobulin having a first T4 /T3 ratio substantially greater than 2.6 and the durations of the denaturing and defatting steps are selected in correlation with said precipitating step duration to lower said T4 /T3 value greater than 2.6 to a final value in the range of 2.6-3.4 and absolute levels of not less than about 0.7 μ
g/mg liothyronine and not less than about 2.1 μ
g/mg levothyroxine.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
-
12. A process for the production of thyroglobulin comprising:
-
(a) digesting hog thyroid glands by adding ground hog thyroid glands to an aqueous saline solution of NaCl and sodium salicylate at a temperature of about 0°
-5°
C. and for a time period of about 16-24 hours;(b) following digestion, separating the resultant mixture into waste tissue and an aqueous solution of thyroglobulin extract; (c) heating the extract to about 46°
C., adjusting the pH thereof to about 4.4, and then heating the extract to about 86°
C., wherein the total time required for the performance of stage (c) is about 0.5 hour;(d) denaturing and precipitating the extract by holding the filtrate at a temperature of about 86°
C. for a time period of about 3 hours;(e) cooling the extract to a temperature of about 60°
C. and, while cooling, filtering the extract to obtain a filter cake of wet crude thyroglobulin;(f) forming a slurry of said wet crude thyroglobulin with alcohol and heating the resultant slurry to reflux, thereby defatting the wet thyroglobulin by extraction, wherein extraction is performed for a time period of about 1.5 hours; (g) filtering the slurry following extraction to again obtain a filter cake of once-extracted thyroglobulin; (h) forming a slurry by mixing once-extracted thyroglobulin with alcohol and again performing extraction in accordance with stage (f); (i) filtering the extracted slurry obtained from stage (h) to again obtain a filter cake of wet defatted thyroglobulin; and (j) drying said defatted thyroglobulin for a sufficient time period at a temperature of 60°
-70°
C. - View Dependent Claims (18)
-
-
13. A process for the production of thyroglobulin having a thyroxine-to-triiodothyronine (T4 /T3) ratio about 2.6-3.4 from a mammalian thyroglobulin extract, comprising:
-
(a) precipitating said thyroglobulin extract by adjusting pH and by heating for a time sufficient to provide a T4 /T3 ratio substantially greater than 2.6; (b) determining by parametric experiments the time of subsequent denaturing and defatting treatments of the resultant thyroglobulin effective to denature and defat the thyroglobulin and to lower said T4 /T3 ratio greater than 2.6 to a value in the range of 2.6-3.4 and absolute levels of not less than about 0.7 μ
g/mg liothyronine and not less than about 2.1 μ
g/mg levothyroxine;(c) denaturing thyroglobulin by heating to a temperature higher than the temperature of precipitation and maintaining the higher temperature in accordance with the time determined in step (b); and (d) defatting thyroglobulin by extraction with alcohol under reflux in accordance with the time determined in step (b). - View Dependent Claims (14)
-
-
15. A process for the production of thyroglobulin from thyroglobulin extract produced from the saline digestion of hog thyroid glands, comprising:
-
(a) precipitating thyroglobulin by pH adjustment of said thyroglobulin extract; (b) denaturing said thyroglobulin extract by heating; and (c) defatting thyroglobulin by extraction with alcohol; wherein the durations of steps (b) and (c) are selected to adjust for an increase in T4 /T3 ratio occurring during step (a) and wherein when the process is repeated at least 60% of the process runs yield defatted thyroglobulin product with a T4 /T3 ratio of about 2.6-3.4 and absolute levels of not less than about 0.7 μ
g/mg liothyronine and not less than about 2.1 μ
g/mg levothyroxine.
-
-
16. A process for the production of thyroglobulin from thyroglobulin extract produced from the saline digestion of hot thyroid glands, comprising:
-
(a) precipitating thyroglobulin by pH adjustment of said thyroglobulin extract; (b) denaturing said thyroglobulin extract and heating; and (c) defatting thyroglobulin by extraction with alcohol; wherein the duration of steps (b) and (c) are sufficient to decrease the T4 /T3 ratio of the thyroglobulin and wherein when the process is repeated at least 60% of the process runs yield a defatted thyroglobulin product with a T4 /T3 ratio of about 2.6-3.4 and absolute levels of not less than about 0.7 μ
g/mg liothyronine and not less than about 2.1 μ
g/mg levothyroxine.
-
-
17. A process for the production of thyroglobulin having a thyroxine-to-triiodothyronine (T4 /T3) ratio of about 2.6-3.4 from denatured mammalian thyroglobulin obtained having a T4 /T3 ratio substantially greater than 2.6, comprising:
defatting thyroglobulin by extraction with alcohol for a time sufficient to achieve a T4 /T3 ratio in the defatted thyroglobulin of about 2.6-3.4 and absolute levels of not less than about 0.7 μ
g/mg liothyronine and not less than about 2.1 μ
g/mg levothyroxine, said time having been determined by parametric experiments varying defatting time for a given T4 /T3 ratio in said denatured mammalian thyroglobulin.
Specification