Electrochemical detector

• US 5,100,530 A
• Filed: 08/16/1990
• Issued: 03/31/1992
• Est. Priority Date: 09/15/1989
• Status: Expired due to Fees

• Current Assignee: Hewlett-Packard Company (HP Inc.)
• Original Assignee: Hewlett-Packard Company (HP Inc.)
• Inventors: Dorr, Thomas, Linowski, Clemens
• Primary Examiner(s): Nguyen, Nam X.
• Application Number:
  US07/568,099
• Time in Patent Office: 593 Days
• Field of Search: 204/406, 204/412, 422/70
• US Class Current: 204/406
• CPC Class Codes: G01N 27/4163 checking the operation of, or calibrating, the measuring apparatus G01N27/3274, G01N27/4175 and G01N33/0006 take precedence
• CPC Class Codes: G01N 27/49 Systems involving the determination of the current at a single specific value, or small range of values, of applied voltage for producing selective measurement of one or more particular ionic species

Abstract

An electrochemical detector comprising an electrochemical cell (11), a working electrode (1), an auxiliary electrode (2), a reference electrode (3), a potentiostat (4), and a circuit (7,8) for deriving a signal indicative of the current developed at the working electrode additionally comprises means for monitoring and determining the potential of the reference electrode (3). These means comprise an electrometer circuit (9) which can be connected via a switch (S2) to the auxiliary electrode (2) to measure the potential of the reference electrode. During the measurement, the working electrode (1) is disconnected from the circuit (7,8) which is at the same time connected to the electrometer circuit (9) via switch (S1). The invention permits to recognize measuring errors associated with instabilities in the reference electrode potential (FIG. 1).

Claims

1. An electrochemical detector for detecting electroactive substances, comprising:

an electrochemical cell for receiving liquid including the substances to be detected,

a working electrode, an auxiliary electrode, a reference electrode, each of said three electrodes being in conductive connection with the liquid in the electrochemical cell,

a potentiostat for maintaining during electrochemical detection a constant potential difference between the liquid in the electrochemical cell and the working electrode, with the potentiostat being coupled during electrochemical detection to the reference electrode and to the auxiliary electrode, and

signal processing circuitry for deriving an electric signal indicative of the current created at the working electrode by electroactive substances,

in combination with monitoring means for determining and monitoring the potential of the reference electrode, said monitoring means comprising;

disconnecting means for disconnecting the working electrode from the signal processing circuitry

grounding means for applying a predetermined potential to the end of the reference electrode opposite to the end in contact with the electrochemical cell,

an electrometer circuit being operative to perform a substantially currentless measurement of the potential of the reference electrode, and

switching means for disconnecting the auxiliary electrode from the potentiostat and for connecting the auxiliary electrode to an electrometer circuit.

2. An electrochemical detector as in claim 1, wherein the disconnecting means are also operative to connect the signal processing circuitry to an output of the electrometer circuit when the working electrode is disconnected from the signal processing circuitry.

3. An electrochemical detector as in claim 1, wherein the measured potential of the reference electrode is displayed to a user.

4. An electrochemical detector as in claim 1, wherein the potential of the reference electrode is capable of being measured several times over a period of time such as to detect any drift of this potential, and wherein electrochemical detection is only enabled if the detected drift is below a predetermined value.

5. An electrochemical detector as in claim 1 wherein:

the potentiostat comprises an operational amplifier having a non-inverting input connected to a source for providing an adjustable input voltage, and an inverting input connected to the reference electrode,

the grounding means comprise a grounding-means switch arranged between the output of the operational amplifier and the reference electrode,

said grounding-means switch being opened during electrochemical detection, and

said grounding-means switch being closed and the input voltage set to 0 volt during measurement of the potential of the reference electrode.

6. An electrochemical detector as in claim 5, wherein the disconnecting means, the switching means and the grounding means switch comprise reed relays.

7. An electrochemical detector as in claim 1, wherein during measurement of the potential of the reference electrode, the reference electrode is disconnected from the potentiostat and directly connected to a fixed potential.

8. The electrochemical detector as in claim 1 adapted to receive said substances to be detected from the effluent of the separation column of a liquid chromatograph.

9. The electrochemical detector as in claim 8, wherein the reference electrode is a redox couple and wherein the anions of the redox couple are provided by the mobile phase of the liquid chromatograph.