Antibody matrix device and method for evaluating immune status
First Claim
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1. An immunoassay device comprising:
- a. support having a substantially planar surface;
b. an array of small, closely-spaced, discrete, antibody-coated areas on the support surface, the array containing antibody coated areas each area individually containing one of the following antibodies;
a. antibody specific for surface antigen present on all mononuclear leukocytes;
b. antibody specific for surface antigen present on all B lymphocytes;
c. antibody specific for surface antigens present on all T lymphocytes;
d. antibody specific for surface antigen present on helper/inducer T lymphocytes;
e. antibody specific for surface antigen present on suppressor/cytotoxic T lymphocytes; and
f. antibody specific for surface antigen present on monocytes; and
c. a cover spaced from the support surface and positioned over the array of antibody coated areas,the cover having at least one aperture communicating with the interior of the chamber for introducing a suspension of leukocytes into the chamber, and,at least one additional aperture in the cover also communicating with the interior of the chamber to allow air to escape upon introduction of the sample into the chamber.
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Abstract
An antibody matrix immunoassay device for determination of number and proportion of subpopulations of leukocytes is described. The device can be used to evaluate and monitor the immune status of an individual and to diagnose and monitor the progression of AIDS.
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Citations
18 Claims
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1. An immunoassay device comprising:
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a. support having a substantially planar surface; b. an array of small, closely-spaced, discrete, antibody-coated areas on the support surface, the array containing antibody coated areas each area individually containing one of the following antibodies; a. antibody specific for surface antigen present on all mononuclear leukocytes; b. antibody specific for surface antigen present on all B lymphocytes; c. antibody specific for surface antigens present on all T lymphocytes; d. antibody specific for surface antigen present on helper/inducer T lymphocytes; e. antibody specific for surface antigen present on suppressor/cytotoxic T lymphocytes; and f. antibody specific for surface antigen present on monocytes; and c. a cover spaced from the support surface and positioned over the array of antibody coated areas, the cover having at least one aperture communicating with the interior of the chamber for introducing a suspension of leukocytes into the chamber, and, at least one additional aperture in the cover also communicating with the interior of the chamber to allow air to escape upon introduction of the sample into the chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 15)
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12. An immunoassay device, comprising:
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a. a support having a substantially planar surface; b. a rectangular array of antibody-coated spots on the planar surface, the array of antibody-coated spots containing a spot or set of spots containing antibodies specific for β
2 microglobulin, a spot or set of spots containing antibody specific for immunoglobulin G, a spot or set of spots containing antibody specific for CD3 antigen, a spot or set of spots containing antibody specific for CD4 antigen, a spot or set of spots containing antibody specific for CD8 antigen, and a spot or set of spots containing antibody specific for OKM1 antigen;c. walls extending upwardly from the support surface and located at the perimeter of each array of antibody-coated spots; and d. a cover mounted upon the walls thereby forming an enclosed chamber over each array, the cover having a sample injection aperture communicating with the chamber space and a plurality of apertures also communicating with the chamber space to permit air to escape. - View Dependent Claims (13, 14)
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16. An immunoassay device for monitoring the immune status of an individual infected with HIV, or diagnosed as having AIDS or ARC, comprising:
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(i) a support having a substantially planar surface; (ii) a rectangular array of antibody-coated spots on the planar surface, the array of antibody-coated spots containing antibodies specific for surface antigen present on leukocytes T lymphocytes, helper/inducer T lymphocytes and suppressor/cytotoxic T lymphocytes; (iii) walls extending upwardly from the support surface and located at the perimeter of each array of antibody-coated spots; and (iv) a cover mounted upon the walls, thereby forming an enclosed chamber over each array, the cover having a sample injection aperture communicating with the chamber and a plurality of apertures also communicating with the chamber to permit air to escape; wherein the support, walls and cover are made of glass or plastic. - View Dependent Claims (17, 18)
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Specification