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Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system

  • US 5,102,797 A
  • Filed: 05/26/1989
  • Issued: 04/07/1992
  • Est. Priority Date: 05/26/1989
  • Status: Expired due to Fees
First Claim
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1. A method for the insertion of foreign DNA into the genome of a recipient bacterium by introduction of a combined plasmid containing a transposable cassette and a transposase encoding sequence which is competent to transpose the cassette, said method comprising the steps of forming the combined plasmid from a carrier plasmid and a functions plasmid by homologous recombination wherein the recombination occurs between overlapping segments of a sselectable marker such that recombination results in the construction of a functional selectable marker.

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