Fixative composition for fixing blood smears to slides

• US 5,104,640 A
• Filed: 03/17/1989
• Issued: 04/14/1992
• Est. Priority Date: 03/17/1989
• Status: Expired due to Term

• Current Assignee: Wesco Incorporated (HF HOLDINGS INC)
• Original Assignee: Wesco Incorporated (HF HOLDINGS INC)
• Inventors: Stokes, Barry O.
• Primary Examiner(s): Lacey, David L.
• Assistant Examiner(s): DALEY, THOMAS
• Application Number:
  US07/325,524
• Time in Patent Office: 1,124 Days
• Field of Search: 436/174, 436/175, 424/3, 420/40
• US Class Current: 435/40.51
• CPC Class Codes: G01N 1/30 Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
• CPC Class Codes: G01N 2001/305 Fixative compositions
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

A fixative solution for fixing biological smears, such as blood smears, to a slide for subsequent staining of the smear, which can be used in automated slide staining equipment, includes a fixative liquid, a stabilizing agent to reduce water spotting problems during fixing and/or to stabilize cellular components of the smear, and a solubilizing agent for maintaining the stabilizing agent in solution in the solubilizing agent after evaporation of the fixative liquid to thereby prevent formation of the solid residue normally formed by the stabilizing agent upon evaporation of the fixative liquid. The invention also includes the method fo preventing formation of a solid residue upon evaporation of the fixative liquid from a fixative liquid-stabilizing agent solution by the addition of a solubilizing agent thereto. The mixture of the stabilizing agent and solubilizing agent can be made as a concentrated additive to be added to a fixative liquid prior to use. The preferred stabilizing agent is polyvinyl pyrrolidone and the preferred solubilizing agent is ethylene glycol.

Claims

1. A substantially nonaqueous fixative composition for fixing a biological smear on a slide for subsequent staining of the smear, comprising a fixative liquid;

a stabilizing agent that would in the absence of a solubilizing agent form a solid residue upon evaporation of the fixative liquid, said stabilizing agent reducing water spotting artifacts produced during the adhering of a blood smear; and

a solubilizing agent for maintaining the stabilizing agent in solution upon evaporation of the fixative liquid, said solubilizing agent making up from about 10% to about 30% by volume of the fixative composition, whereby said composition causes a blood smear to adhere to a slide so that the smear will not wash off during subsequent staining of the smear, said composition being substantially nonaqueous so as not to interfere with the reduction in water spotting problems effected by the stabilizing agent.

2. A substantially nonaqueous fixative composition according to claim 1, wherein the stabilizing agent makes up to about 6% by weight of the fixative solution.

3. A substantially nonaqueous fixative composition according to claim 1, wherein the stabilizing agent makes up to about 2% by weight of the fixative solution and the solubilizing agent makes up to about 10% by volume of the fixative solution.

4. A substantially nonaqueous fixative composition according to claim 1, wherein the fixative liquid is methanol, the stabilizing agent is polyvinyl pyrrolidone which makes up to about 2% by weight of the fixative solution, and the solubilizing agent is ethylene glycol which makes up to about 10% by volume of the fixative solution.

5. A substantially nonaqueous fixative composition according to claim 1, wherein the stabilizing agent is selected from the group consisting of polyvinyl pyrrolidone, polyethylene glycol, a dye, or a combination thereof.

6. A substantially nonaqueous fixative composition according to claim 1, wherein the solubilizing agent is selected from the group consisting of ethylene glycol;

diethylene glycol;

propylene glycol;

a butanediol;

methyl, ethyl, and butyl ethers of ethylene glycol and diethylene glycol;

the acetate esters of ethylene glycol, diethylene glycol, and the ether derivatives thereof;

furfuryl alchohol;

tetrahydrofurfuryl alcohol;

diacetone alcohol;

N methyl 2-pyrrolidinone;

N, N-dimethyformamide;

γ

butyrolactone;

1-4 dioxane; and

dimethyl sulfoxide.

7. An additive composition for addition to a fixative liquid that causes a blood smear to adhere to a slide, said additive composition comprising a stabilizing agent making up to about 50% by volume of the additive composition and reducing water spotting artifacts produced during the adhering of the blood smear;

and a solubilizing agent for maintaining the stabilizing agent in solution so as to prevent the stabilizing agent from forming a solid residue upon evaporation of the fixative liquid, the resulting composition being substantially nonaqueous after the addition of said additive composition so as not to interfere with the reduction in water spotting problems effected by the stabilizing agent, said additive composition when diluted with a fixative liquid will cause a blood smear to adhere to a slide so as not to wash off during subsequent application of stains to the smear.

8. An additive composition according to claim 7, wherein the stabilizing agent makes up to about 50% by weight of the additive.

9. An additive composition according to claim 7, wherein the stabilizing agent is polyvinyl pyrrolidone and the solubilizing agent is ethylene glycol.

10. An additive composition according to claim 7, wherein the additive comprises between 150 and 500 grams of stabilizing agent for each 500 grams of solubilizing agent.

11. An additive composition according to claim 10, wherein the stabilizing agent is polyvinyl pyrrolidone and the solubilizing agent is ethylene glycol.

12. An additive composition according to claim 7, wherein the stabilizing agent is selected from the group consisting of polyvinyl pyrrolidone, polyethylene glycol, a dye, or a combination thereof.

13. An additive composition according to claim 7, wherein the solubilzing agent is selected from the group consisting of ethylene glycol;

diethylene glycol;

propylene glycol;

a butanediol;

methyl, ethyl, and butyl ethers of ethylene glycol and diethylene glycol;

the acetate esters of ethylene glycol, diethylene glycol, and the ether derivatives thereof;

furfuryl alcohol;

tetrahydrofurfuryl alcohol;

diacetone alcohol;

N methyl 2-pyrrolidinone;

N, N-dimethyformamide;

γ

butyrolactone;

1-4 dioxane; and

dimethyl sulfoxide.

14. A substantially nonaqueous fixative composition, comprising 64-99% of a fixative liquid;

up to 6% of a stabilizing agent that would in the absence of a solubilizing agent form a solid residue upon evaporation of the fixative liquid, said stabilizing agent reducing water spotting artifacts produced during the adhering of the blood smear; and

up to 30% of a solubilizing agent, whereby said composition causes a blood smear to adhere to a slide so that the smear will not wash off during subsequent staining of the smear, said composition being substantially nonaqueous so as not to interfere with the reduction in water spotting problems effected by the stabilizing agent.

15. A substantially nonaqueous fixative composition, comprising 88-99% of a fixative liquid;

up to 2% of a stabilizing agent that would in the absence of a solubilizing agent form a solid residue upon evaporation of the fixative liquid, said stabilizing agent reducing water spotting artifacts produced during the adhering of a blood smear; and

up to 30% of a solubilizing agent, whereby said composition causes a blood smear to adhere to a slide so that the smear will not wash off during subsequent staining of the smear, said composition being substantially nonaqueous so as not to interfere with the reduction in water spotting problems effected by the stabilizing agent.