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Fixative composition for fixing blood smears to slides

  • US 5,104,640 A
  • Filed: 03/17/1989
  • Issued: 04/14/1992
  • Est. Priority Date: 03/17/1989
  • Status: Expired due to Term
First Claim
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1. A substantially nonaqueous fixative composition for fixing a biological smear on a slide for subsequent staining of the smear, comprising a fixative liquid;

  • a stabilizing agent that would in the absence of a solubilizing agent form a solid residue upon evaporation of the fixative liquid, said stabilizing agent reducing water spotting artifacts produced during the adhering of a blood smear; and

    a solubilizing agent for maintaining the stabilizing agent in solution upon evaporation of the fixative liquid, said solubilizing agent making up from about 10% to about 30% by volume of the fixative composition, whereby said composition causes a blood smear to adhere to a slide so that the smear will not wash off during subsequent staining of the smear, said composition being substantially nonaqueous so as not to interfere with the reduction in water spotting problems effected by the stabilizing agent.

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