Fixative composition for fixing blood smears to slides
First Claim
1. A substantially nonaqueous fixative composition for fixing a biological smear on a slide for subsequent staining of the smear, comprising a fixative liquid;
- a stabilizing agent that would in the absence of a solubilizing agent form a solid residue upon evaporation of the fixative liquid, said stabilizing agent reducing water spotting artifacts produced during the adhering of a blood smear; and
a solubilizing agent for maintaining the stabilizing agent in solution upon evaporation of the fixative liquid, said solubilizing agent making up from about 10% to about 30% by volume of the fixative composition, whereby said composition causes a blood smear to adhere to a slide so that the smear will not wash off during subsequent staining of the smear, said composition being substantially nonaqueous so as not to interfere with the reduction in water spotting problems effected by the stabilizing agent.
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Accused Products
Abstract
A fixative solution for fixing biological smears, such as blood smears, to a slide for subsequent staining of the smear, which can be used in automated slide staining equipment, includes a fixative liquid, a stabilizing agent to reduce water spotting problems during fixing and/or to stabilize cellular components of the smear, and a solubilizing agent for maintaining the stabilizing agent in solution in the solubilizing agent after evaporation of the fixative liquid to thereby prevent formation of the solid residue normally formed by the stabilizing agent upon evaporation of the fixative liquid. The invention also includes the method fo preventing formation of a solid residue upon evaporation of the fixative liquid from a fixative liquid-stabilizing agent solution by the addition of a solubilizing agent thereto. The mixture of the stabilizing agent and solubilizing agent can be made as a concentrated additive to be added to a fixative liquid prior to use. The preferred stabilizing agent is polyvinyl pyrrolidone and the preferred solubilizing agent is ethylene glycol.
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Citations
15 Claims
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1. A substantially nonaqueous fixative composition for fixing a biological smear on a slide for subsequent staining of the smear, comprising a fixative liquid;
- a stabilizing agent that would in the absence of a solubilizing agent form a solid residue upon evaporation of the fixative liquid, said stabilizing agent reducing water spotting artifacts produced during the adhering of a blood smear; and
a solubilizing agent for maintaining the stabilizing agent in solution upon evaporation of the fixative liquid, said solubilizing agent making up from about 10% to about 30% by volume of the fixative composition, whereby said composition causes a blood smear to adhere to a slide so that the smear will not wash off during subsequent staining of the smear, said composition being substantially nonaqueous so as not to interfere with the reduction in water spotting problems effected by the stabilizing agent. - View Dependent Claims (2, 3, 4, 5, 6)
- a stabilizing agent that would in the absence of a solubilizing agent form a solid residue upon evaporation of the fixative liquid, said stabilizing agent reducing water spotting artifacts produced during the adhering of a blood smear; and
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7. An additive composition for addition to a fixative liquid that causes a blood smear to adhere to a slide, said additive composition comprising a stabilizing agent making up to about 50% by volume of the additive composition and reducing water spotting artifacts produced during the adhering of the blood smear;
- and a solubilizing agent for maintaining the stabilizing agent in solution so as to prevent the stabilizing agent from forming a solid residue upon evaporation of the fixative liquid, the resulting composition being substantially nonaqueous after the addition of said additive composition so as not to interfere with the reduction in water spotting problems effected by the stabilizing agent, said additive composition when diluted with a fixative liquid will cause a blood smear to adhere to a slide so as not to wash off during subsequent application of stains to the smear.
- View Dependent Claims (8, 9, 10, 11, 12, 13)
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14. A substantially nonaqueous fixative composition, comprising 64-99% of a fixative liquid;
- up to 6% of a stabilizing agent that would in the absence of a solubilizing agent form a solid residue upon evaporation of the fixative liquid, said stabilizing agent reducing water spotting artifacts produced during the adhering of the blood smear; and
up to 30% of a solubilizing agent, whereby said composition causes a blood smear to adhere to a slide so that the smear will not wash off during subsequent staining of the smear, said composition being substantially nonaqueous so as not to interfere with the reduction in water spotting problems effected by the stabilizing agent.
- up to 6% of a stabilizing agent that would in the absence of a solubilizing agent form a solid residue upon evaporation of the fixative liquid, said stabilizing agent reducing water spotting artifacts produced during the adhering of the blood smear; and
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15. A substantially nonaqueous fixative composition, comprising 88-99% of a fixative liquid;
- up to 2% of a stabilizing agent that would in the absence of a solubilizing agent form a solid residue upon evaporation of the fixative liquid, said stabilizing agent reducing water spotting artifacts produced during the adhering of a blood smear; and
up to 30% of a solubilizing agent, whereby said composition causes a blood smear to adhere to a slide so that the smear will not wash off during subsequent staining of the smear, said composition being substantially nonaqueous so as not to interfere with the reduction in water spotting problems effected by the stabilizing agent.
- up to 2% of a stabilizing agent that would in the absence of a solubilizing agent form a solid residue upon evaporation of the fixative liquid, said stabilizing agent reducing water spotting artifacts produced during the adhering of a blood smear; and
Specification