Shortened phosphoglycerate kinase promoter
First Claim
Patent Images
1. A shortened promoter of the PGK gene, wherein said promoter comprises about 165 to about 404 base pairs of the proximal end of the 5'"'"' region of the PGK gene, said promoter providing improved expression of polypeptides under its control as compared to said expression provided by the full-length PGK promoter.
2 Assignments
0 Petitions
Accused Products
Abstract
A shortened PGK promoter ranging from 165 to 404 base pairs of the Saccharomyces PGK promoter is taught. The promoter is operably linked to heterologous genes and improves the expression thereof relative to the full length PGK promoter.
20 Citations
12 Claims
- 1. A shortened promoter of the PGK gene, wherein said promoter comprises about 165 to about 404 base pairs of the proximal end of the 5'"'"' region of the PGK gene, said promoter providing improved expression of polypeptides under its control as compared to said expression provided by the full-length PGK promoter.
- 3. An expression vehicle for use in recombinant DNA which is capable of replicating and expressing genetic information and having the promoter base pair sequence length of about 165 to about 404 base pairs of the proximal end of the 5'"'"' region of the PGK gene, said promoter controlling the expression of a structural polypeptide.
- 4. A expression vehicle for use in recombinant DNA which is capable of replicating and expressing genetic information and having the promoter base pair sequence length of about 165 to about 404 base pairs of the proximal end of the 5'"'"' region of the PGK gene operably linked to a secretion signal, said promoter and secretion signal controlling the expression and secretion of a structural polypeptide.
- 8. A method of producing a desired polypeptide comprising culturing a host cell transformed by an expression vehicle having the promoter base pair sequence length of about 165 to about 404 base pairs of the proximal end of the 5'"'"' region of the PGK gene operably linked to a secretion signal, said promoter and secretion signal controlling the expression and secretion of said polypeptide, under conditions selected to produce and secrete said polypeptide into the culture medium.
Specification