Photobioreactor

• US 5,104,803 A
• Filed: 03/03/1988
• Issued: 04/14/1992
• Est. Priority Date: 03/03/1988
• Status: Expired due to Term

• Current Assignee: Martek Biosciences Corporation (DSM-Firmenich AG)
• Original Assignee: Martek Biosciences Corporation (DSM-Firmenich AG)
• Inventors: Delente, Jacques
• Primary Examiner(s): Warden, Robert J.
• Assistant Examiner(s): Beisner, William H.
• Application Number:
  US07/163,800
• Time in Patent Office: 1,503 Days
• Field of Search: 435/257, 435/287, 435/289, 435/290, 435/313, 435/314, 435/316, 435/284, 435/285, 422/186, 422/186.3, 47/1.4, 250/432 R, 250/494.1, 362/101, 362/240, 362/267, 362/375
• US Class Current: 435/292.1
• CPC Class Codes: C12M 21/02 Photobioreactors culturing algae A01G33/00, A01H4/001, C12N1/12
• CPC Class Codes: C12M 31/10 by light emitting elements located inside the reactor, e.g. LED or OLED
• CPC Class Codes: C12M 41/02 of foam foam prevention during gasification of liquids B01D19/02
• CPC Class Codes: C12M 41/44 of volume or liquid level

Abstract

The present invention relates to a photobioreactor for the cultivation of photosynthetic micoorganisms having at least one light bank substantially totally immersible in the liquid microbial culture contained in the photobioreactor so that substantially all of the emitted light is absorbed in the culture. The light bank comprises a plurality of light tubes in substantially close proximity to each other, electrical leads extending from the light tubes to a source of electric power and an enclosure means enclosing at least the portions of the electrical leads into the lead tubes to render such points impervious to the liquid microbial culture. The light bank serves as a light source as well as may be arranged to form channels for the circulation of the liquid culture within the photobioreactor.

Claims

1. A photobioreactor comprising:

(a) a tank for containing a liquid microbial culture at a preselected operating level within said tank;

(b) a plurality of modular light banks within said tank, each of said plurality of modular light banks comprising;

(i) a plurality of light tubes, wherein each of said light tubes has ends with electrical contacts, a longitudinal axis and an outer light emitting surface, said light tubes being arranged in close proximity to one another so that the outer light emitting surfaces are separated by a predetermined distance and the longitudinal axes of the light tubes are substantially parallel and lie generally in a plane;

(ii) electrical leads connected to the ends of each of said light tubes for connection to a source of electrical power; and

(iii) an enclosure means forming a water-tight compartment surrounding the light tubes, wherein said enclosure means comprises;

generally flat, transparent side walls which are substantially parallel to one another and to the plane of the longitudinal axes of the light tubes, and which are in close proximity to the light tubes,

end walls extending in the direction of the longitudinal axes of the light tubes and in sealed attachment to the side walls,

a bottom wall in sealed attachment to the side walls and the end walls, and

a top wall, with at least one aperture through which the electrical leads pass, in sealed attachment to the side walls and end walls; and

(c) support means constructed and arranged so as to support and position said modular light banks within said tank in adjacent, spaced apart and substantially parallel relation to each other with a periodic preselected distance between adjacent banks such that when said tank is filled with a liquid microbial culture at the preselected operating level, substantially the entire light emitting surfaces of said light tubes are immersed in said culture and substantially all of the light emitted from said light tubes is transmitted into the culture.

2. The photobioreactor as set forth in claim 1 in which said light tubes are fluorescent tube lamps.

3. The photobioreactor as set forth in claim 1 in which the predetermined distance between the outer light emitting surfaces of the light tubes within the modular light banks is from about 0 to about 3 centimeters.

4. The photobioreactor as set forth in claim 1, further including a gas introduction means positioned and arranged so as to introduce a sparging gas between at least some of the modular light banks.

5. The photobioreactor as set forth in claim 1, in which the preselected distance between adjacent modular light banks is from about 1 to about 3 centimeters and in which the predetermined spacing between the outer light emitting surfaces of the light tubes within the modular light banks is from about 0 to about 3 centimeters.

6. The photobioreactor as set forth in claim 1, wherein said support means is further constructed and arranged so as to cover a top opening of the tank.

7. The photobioreactor as set forth in claim 1, wherein the side walls of said enclosure means have sufficient flexibility so that when exposed to hydrostatic pressure in the photobioreactor, said side walls will contract and partially conform to a shape of said light tubes.

8. The photobioreactor as set forth in claim 1, wherein the side walls of said enclosure means have a thickness ranging from about 1/16" to about 1/2".

9. The photobioreactor as set forth in claim 8, wherein the side walls of said enclosure means have a thickness of 1/16".

10. The photobioreactor as set forth in claim 1, wherein said side walls, said end walls, said bottom wall, and said top wall are made of methyl methacrylate or polycarbonate.

11. The photobioreactor as set forth in claim 1, wherein said tank has a plurality of side walls and said support means is further constructed and arranged so as to be in communication with said plurality of tank side walls.

12. The photobioreactor as set forth in claim 1, further comprising an electrical supply connected to said electrical leads, temperature control means constructed and arranged so as to control the temperature of the culture within said tank, gas supply means constructed and arranged so as to introduce gas into said tank, and antifoaming means constructed and arranged so as to prevent the formation of foam within said tank.

13. The photobioreactor as set forth in claim 12, wherein said temperature control means comprises a heating unit and cooling unit in fluid communication with at least one water jacket located on at least one side of said tank.

14. A light bank for a photobioreactor which comprises:

(i) a plurality of light tubes, where each of said light tubes has ends with electrical contacts, a longitudinal axis and an outer light emitting surface, said light tubes being arranged in close proximity to one another so that the outer light emitting surfaces are separated by a predetermined distance and the longitudinal axes of the light tubes are substantially parallel and lie generally in a plane;

(ii) electrical leads connected to the electrical contacts of each of said light tubes for connection to a source of electrical power; and

(iii) an enclosure means forming a water-tight compartment surrounding the light tubes, wherein said enclosure means comprises;

generally flat, transparent side walls which are substantially parallel to one another and to the plane of the longitudinal axes of the light tubes, and which are in close proximity of the light tubes,

end walls extending in the direction of the longitudinal axes of the light tubes and in sealed attachment to the side walls,

a bottom wall in sealed attachment to the side walls and the end walls, and

a top wall, with at least one aperture through which the electrical leads pass, in sealed attachment to the side walls and end walls.

15. The light bank for a photobioreactor as set forth in claim 14 wherein the side walls of said enclosure means have sufficient flexibility so that when exposed to hydrostatic pressure in the photobioreactor, said side walls will contact and partially conform to a shape of said light tubes.

16. The light bank for a photobioreactor as set forth in claim 14, in which said light tubes are fluorescent tube lamps.

17. The light bank for a photobioreactor as set forth in claim 14, in which the predetermined distance between the outer light emitting surfaces of the light tubes within the light banks is from about 0 to about 3 centimeters.

18. The light bank for a photobioreactor as set forth in claim 14, wherein said at least one aperture comprises two apertures.

19. The light bank for a photobioreactor as set forth in claim 14, wherein the side walls of said enclosure means have a thickness ranging from about 1/16" to about 1/2".

20. The light bank for a photobioreactor as set forth in claim 19, wherein the side walls of said enclosure means have a thickness of 1/16".

21. The light bank for a photobioreactor as set forth in claim 14, wherein said side walls, said end walls, said bottom wall, and said top wall are made of methyl methacrylate or polycarbonate.

22. A photobioreactor comprising:

(a) a tank for containing a liquid microbial culture at a preselected operating level within said tank;

(b) a plurality of compartment forming means positioned within said tank in adjacent, parallel relation to each other and having a periodic spacing with respect to each other, each of said compartment forming means comprising generally flat optically transparent side walls;

(c) a light bank within said each of said compartment forming means, said light bank comprising a plurality of light tubes supported and positioned within said compartment forming means in substantially close proximity with each other;

(d) means for sealing said compartment forming means against fluid communication with the interior of said tank; and

(e) each of said compartment forming means being positioned within said tank such that substantially all light emitted from said light tubes is transmitted to said culture when said tank contains said culture at said preselected operating level.