Cell assay device used in a microphysiometer

• US 5,104,804 A
• Filed: 06/04/1990
• Issued: 04/14/1992
• Est. Priority Date: 06/04/1990
• Status: Expired due to Fees

• Current Assignee: Molecular Devices
• Original Assignee: Molecular Devices
• Inventors: Libby, Jeffrey M., Miller, Donald L., Schwartz, Henry L., Humphries, Gillian M. K.
• Primary Examiner(s): Lacey, David L.
• Assistant Examiner(s): CHAN, KWONG
• Application Number:
  US07/532,571
• Time in Patent Office: 680 Days
• Field of Search: 435/288, 435/817, 435/285, 435/182, 435/287, 435/291, 204/403
• US Class Current: 435/287.1
• CPC Class Codes: C12M 25/04 in combination with well or multiwell plates, i.e. culture inserts
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

This invention encompasses porous microchambers which contain cells and permit liquid to flow in and out of the chamber while retaining cells within the chamber. These porous microchambers serve as disposable devices for placing cells in a microflowchamber so that properties of the cells within the porous microchamber can be measured.

Claims

1. A device for removably placing cells in a microflowchamber of a microphysiometer comprising:

(a) an outer sleeve having a top and bottom opening wherein the bottom opening of said outer sleeve is covered with a porous membrane;

(b) an inner sleeve fitting within the outer sleeve and having a top and bottom opening wherein the bottom opening of said outer sleeve is covered with a porous membrane; and

(c) a spacer means between the porous membranes of the inner and outer sleeves which defines and opening and which together with the porous membranes defines a porous microchamber wherein cells are maintained in the porous microchamber when liquid flows through the porous membranes wherein the porous microchamber has a volume of between 10 nanoliters and 10 microliters.

2. A device according to claim 1 wherein the spacer means is a continuous strip of thin plastic material fitting an inner wall of the outer sleeve and defining said spacer means defined opening.

3. A device according to claim 2 wherein the spacer means is integral with the outer or inner sleeve.

4. A device according to claim 3 wherein the opening defined by the spacer means is covered with a polymer matrix which entraps cells.

5. A device according to claim 4 wherein the polymer matrix is a collagen sponge.

6. In combination with the device of claims 1, 2, 3, 4 or 5, a plunger with a top and bottom surface which has openings for directing a stream of liquid through the plunger and which fits into the inner sleeve and the bottom surface of the plunger contacts the porous membrane of the inner sleeve;

a silicone electrode which contacts the outer surface of the porous membrane of the outer sleeve, wherein when the plunger is pressed a seal is formed between the porous membranes, the spacer means, the plunger and the silicon electrode to define a microflowchamber where liquids are flowed into and out of the microflowchamber through the openings in the plunger wherein the porous microchamber is contained within the microflowchamber.

7. The combination according to claim 6 wherein the plunger has a spring retaining means.