Cryopreservation container of animal cell

• US 5,105,627 A
• Filed: 04/20/1990
• Issued: 04/21/1992
• Est. Priority Date: 04/20/1990
• Status: Expired due to Fees

• Current Assignee: Nihon Freezer Co Ltd
• Original Assignee: Nihon Freezer Co Ltd
• Inventors: Kurita, Susumu
• Primary Examiner(s): Bennett, Henry A.
• Assistant Examiner(s): KILNER, CHRISTOPHER B
• Application Number:
  US07/512,201
• Time in Patent Office: 732 Days
• Field of Search: 62/62, 62/337, 62/371, 62/457.1, 62/457.2, 62/457.9
• US Class Current: 62/62
• CPC Class Codes: A01N 1/02 Preservation of living parts
• CPC Class Codes: A01N 1/0257 Stationary or portable vessels generating cryogenic temperatures
• CPC Class Codes: B65D 81/18 providing specific environment for contents, e.g. temperature above or below ambient with thermal insulation B65D81/38; ice-boxes with cooling means F25D
• CPC Class Codes: B65D 81/382 provided with liquid material between double walls
• CPC Class Codes: C12N 1/04 Preserving or maintaining viable microorganisms immobilised microorganisms C12N11/00
• CPC Class Codes: F25D 2303/0831 the liquid is disposed in the space between the walls of the container
• CPC Class Codes: F25D 2331/804 Boxes
• CPC Class Codes: F25D 3/00 Devices using other cold materials; Devices using cold-storage bodies
• CPC Class Codes: G01N 1/42 Low-temperature sample treatment, e.g. cryofixation

Abstract

A cryopreservation container comprising a bottomed cylindrical hollow container body, a lid which is removably attached to the container body to close the hollow portion thereof, and heat conductivity-retarded liquid enclosed in the hollow portion of the container body.

Claims

1. A cryopreservation container comprising a bottomed hollow container body, said container body including an inner wall portion having an open top forming a hollow container portion, an outer wall portion surrounding said inner wall portion, and a hollow heat conductivity-retarded liquid portion between said inner wall portion and said outer wall portion, a lid for closing said hollow container portion, and heat conductivity-retarded liquid enclosed within said hollow heat conductivity-retarded liquid portion of said container body, and wherein said heat conductivity-retarded liquid comprises a liquid which provides, when ambient temperature of the cryopreservation container is -60°

C.˜

-100°

C., a cooling rate in said container body of -0.5°

C./min.˜

-2°

C./min.

2. The cryopreservation container according to claim 1, wherein said container body, said inner wall portion and said outer wall portion are cylindrically shaped.

3. The cryopreservation container according to claim 2, including a ring member connecting an upper portion of each of said inner wall portion and said outer wall portion to each other.

4. The cryopreservation container according to claim 3, wherein said ring member includes at least one aperture.

5. The cryopreservation container according to claim 4, wherein said at least one aperture is sealed.

6. The cryopreservation container according to claim 1, wherein said lid is removably attached to said container body.

7. The cryopreservation container according to claim 1, wherein said lid comprises a heat insulative solid material.

8. The cryopreservation container according to claim 1, wherein said lid comprises a hollow portion in which heat conductivity-retarded liquid is enclosed.

9. The cryopreservation container according to claim 1, wherein said heat conductivity-retarded liquid comprises a solution of acetone in water.

10. The cryopreservation container according to claim 9, wherein said solution of acetone in water comprises 20˜

40 volume % of acetone in water.

11. The cryopreservation container according to claim 1, wherein said heat conductivity-retarded liquid comprises ethanol.

12. The cryopreservation container according to claim 1, wherein said container body is composed of a synthetic resin.

13. The cryopreservation container according to claim 12, wherein said synthetic resin comprises polyethylene.

14. A combination of a cryopreservation container and an enclosure container in which an animal cell to be frozen and preserved is enclosed together with a cryopreservation medium, said combination comprising:

a cryopreservation container comprising a bottomed hollow container body, said container body including an inner wall portion having an open top forming a hollow container portion, an outer wall portion surrounding said inner wall portion, and a hollow heat conductivity-retarded liquid portion between said inner wall portion and said outer wall portion, a lid for closing said hollow container portion, and heat conductivity-retarded liquid enclosed within said hollow heat conductivity-retarded liquid portion of said container body, wherein said heat conductivity-retarded liquid comprises a liquid which provides, when ambient temperature of the cryopreservation container is -60°

C.˜

-100°

C., a cooling rate in said container body of -0.5°

C./min.˜

-2°

C./min; and

an enclosure container for enclosing an animal cell to be frozen and preserved together with a cryopreservation medium.

15. The combination of a cryopreservation container and an enclosure container according to claim 14, wherein said enclosure container is housed in said hollow container portion of said container body.

16. The combination of a cryopreservation container and an enclosure container according to claim 14, wherein said enclosure container comprises a metal tube.

17. The combination of a cryopreservation container and an enclosure container according to claim 16, wherein said metal tube is composed of aluminum.

18. The combination of a cryopreservation container and an enclosure container according to claim 16, wherein said enclosure container is composed of aluminum alloy.

19. The combination of a cryopreservation container and an enclosure container according to claim 14, further including a cryopreservation medium within said enclosure container.