Microfiltration apparatus with radially spaced nozzles
First Claim
1. Microfiltration apparatus comprising:
- a manifold plate having an array of filtering wells for receiving liquid samples to be filtered;
an array of planar bottom members, wherein each of said wells is closed at its bottom by one of said planar bottom members so as to provide a filter support surface for a filter medium;
a filter disposed on said filter support surface; and
at least one individual filtrate discharge nozzle in fluid communication with each of said filtering wells, each of said discharge nozzles depending from the bottom of each of said planar members at a point radially outward from the sidewall of the filtering well in communication therewith.
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Accused Products
Abstract
The present invention relates to a multiwell apparatus for biological and biochemical analysis which provides for separate collection of filtrate from each well, without cross-contamination therebetween. The present invention provides a manifold plate having an array of filtering wells, each of the wells being closed at its bottom by a planar bottom member providing a filter support surface. A discharge nozzle of passageway depends from each well bottom (planar bottom member) in a direction normal thereto, with one such discharge nozzle or port being provided in fluid communication with each filtering well. Each of these filtrate nozzles or drain ports extends from the lower surface of the planar bottom member to such an extent that it will enter into the well of a conventional multiwell, e.g., microtiter, plate in alignment therewith and mate with the sidewall of the aligned well of the multiwell plate. In this manner, the filtrate can be delivered to the collection well of the multiwell plate as a steady stream, in the manner one would use a pipette, rather than dropwise. The ability to quantitatively collect filtrate into a receiving multiwell plate provides options for improved immunoassays, nucleic acid probe assays and other test procedures.
239 Citations
13 Claims
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1. Microfiltration apparatus comprising:
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a manifold plate having an array of filtering wells for receiving liquid samples to be filtered; an array of planar bottom members, wherein each of said wells is closed at its bottom by one of said planar bottom members so as to provide a filter support surface for a filter medium; a filter disposed on said filter support surface; and at least one individual filtrate discharge nozzle in fluid communication with each of said filtering wells, each of said discharge nozzles depending from the bottom of each of said planar members at a point radially outward from the sidewall of the filtering well in communication therewith. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. Microchromatography apparatus comprising:
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a manifold plate having an array of filtering wells for receiving liquid samples; an array of planar bottom members, wherein each of said wells is closed at its bottom by one of said planar bottom members so as to provide a filter support surface for a filter medium; filter members within a plurality o said filtering wells, each of said filter members being sealed around its periphery to the walls of said wells and resting on said support surface; chromatography medium within said plurality of filtering wells and a porous disc sealed within each of said plurality of wells with said chromatography medium held in a compacted state between said porous disc and said filter member; at least one individual filtrate discharge nozzle in fluid communication with each of said filtering wells, each of said discharge nozzles depending from the bottom of each of sad planar members at a point radially outward from the sidewall of the filtering well in communication therewith; and fluid conduits providing said fluid communication between each filtering well and said at least one discharge nozzle in fluid communication therewith.
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Specification