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Simultaneous immunoassay of two analytes using dual enzyme labelled antibodies

  • US 5,108,896 A
  • Filed: 03/02/1987
  • Issued: 04/28/1992
  • Est. Priority Date: 03/21/1986
  • Status: Expired due to Fees
First Claim
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1. A dual analyte immunoassay process for simultaneously determining first and second analyte ligands present in a liquid sample which comprises:

  • (a) incubating the liquid sample simultaneously with at least one alkaline phosphatase labelled antibody specifically bindable to an epitope of the first analyte ligand and at least one β

    -galactosidase labelled antibody specifically bindable to an epitope of the second analyte ligand so as to form corresponding distinctly labelled antibody-ligand complexes in a liquid phase,(b) separating the distinctly labelled antibody-ligand complexes as a solid phase from the liquid phase,(c) incubating either the solid phase or the liquid phase simultaneously with both o- or p-nitrophenyl-β

    -D-galactoside and phenolphthalein monophosphate, and(d) simultaneously monitoring the production of o- or p-nitrophenol and phenolphthalein and determining the first and second analyte ligands therefrom.

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