Chemiluminescence assay of in vivo inflammation

• US 5,108,899 A
• Filed: 10/31/1989
• Issued: 04/28/1992
• Est. Priority Date: 10/31/1989
• Status: Expired due to Fees

• Current Assignee: Robert C. Allen
• Original Assignee: Exoxemis, Inc.
• Inventors: Allen, Robert C.
• Primary Examiner(s): Kepplinger, Esther L.
• Assistant Examiner(s): Wortman, Donna C.
• Application Number:
  US07/429,105
• Time in Patent Office: 910 Days
• Field of Search: 435/7.21, 435/7.2, 435/7.24, 435/29, 435/25, 435/34, 435/39, 435/968, 436/63, 436/172, 436/503, 436/519, 436/821
• US Class Current: 435/7.21
• CPC Class Codes: G01N 33/5091 for testing the pathological state of an organism
• CPC Class Codes: G01N 33/566 using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
• CPC Class Codes: G01N 33/56972 White blood cells
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: Y10S 435/968 High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
• CPC Class Codes: Y10S 435/975 Kit

Abstract

The presence or amount of in vivo inflammation of a patient is determined by comparing the extent of opsonin receptor expression in vivo on phagocytes of a patient with the maximum opsonin receptor expression inducible on phagocytes of the patient in vitro after stimulation with a receptor expression priming agent. Preferably, the in vivo state of inflammation of a patient is determined by contacting a first portion of a phagocyte containing biological sample from the patient with a opsonified oxidative metabolism stimulating agent capable of elicting metabolic activation and with a chemiluminigenic substrate, contacting a second portion of the biological sample from the patient with an opsonin receptor expression priming agent, an opsonfield oxidative metabolism stimulating agent capable of eliciting metabolic activation and a chemiluminigenic substrate, and then comparing the chemiluminescence response of the first and second portions of the sample as a measure of the immune response potential or state of inflammation of the patient. Phagocyte function is additionally quantitatively evaluated by measuring the phagocyte oxygenation capacity of a maximally opsonin receptor primed and stimulated biological sample of a patient, determining the specific oxygenation capacity per phagocyte in the sample, and comparing the specific oxygenation capacity to a set of controls representing the normal distribution of specific oxygenation established from testing a large population. The phagocyte-specific oxygenation capacity is determined by contacting the sample with an opsonin receptor expression priming agent, an opsonified oxidative metabolism stimulating agent and a chemiluminigenic substrate, measuring the chemiluminescence response of the sample, determining the chemiluminescence response per phagocyte of the sample and comparing the response per phagocyte with that of the normal range of values. Kits and reagents are provided for use in the practice of the disclosed methods.

Claims

1. A method of evaluating the in vivo state of inflammation of a patient, comprising:

a) determining the extent of opsonin receptor expression on phagocytes in a first portion of a phagocyte-containing biological sample of a patient,

b) contacting a second portion of the biological sample with receptor expression priming agent to obtain maximal expression of opsonin receptors on phagocytes in the second portion of the biological sample;

c) determining the extend of opsonin receptor expression on phagocytes in the second portion of the sample; and

then

d) comparing the extent of opsonin receptor expression on the phagocytes of the first and second portions of the sample as a measure of the in vivo state of inflammation of the patient.

2. The method of claim 1 wherein the patient is a human or animal patient and the biological sample comprises a diluted or undiluted biological fluid selected from the group consisting of whole blood, synovial fluid, amniotic fluid, cerebrospinal fluid, peritoneal fluid, pleural fluid, pericardial fluid, and fluids derived therefrom.

3. The method of claim 2 wherein the biological sample is diluted or undiluted whole blood.

4. The method of claim 1 wherein the extent of opsonin receptor expression is determined by contacting the first and second portions of the sample with an opsonified oxidative metabolism stimulating agent and a chemiluminigenic substrate, and then measuring the chemiluminescence response of phagocytes in the first and second portions of the sample as an indication of the extent of receptor expression.

5. The method of claim 4 wherein first and second portions of the sample are contacted with non-rate limiting quantities of the opsonified oxidative metabolism stimulating agent and the chemiluminigenic substrate.

6. The method of claim 5 wherein the second portion of the sample is contacted with a non-rate limiting quantity of the receptor expression priming agent.

7. The method of claim 4 wherein the opsonified oxidative metabolism stimulating agent is coated with one or more of the members of the group consisting of immunoglobulin and complement-derived opsonins.

8. The method of claim 4 wherein the opsonified oxidative metabolism stimulating agent is selected from the group consisting of opsonified attenuated bacteria, opsonified attenuated yeast and opsonified synthetic materials capable of fixing complement or eliciting specific antibody expression.

9. The method of claim 8 wherein the opsonified oxidative metabolism stimulating agent is opsonified zymosan.

10. The method of claim 1 wherein the receptor expression priming agent is selected from the group consisting of C5a, C5a_desArg, N-formyl-methionyl peptides, leukotrienes, platelet activating factor, and myeloid colony stimulating factors.

11. The method of claim 10 wherein the receptor expression priming agent is C5a or C5a_desArg.

12. The method of claim 10 wherein the receptor expression priming agent is an N-formyl-methionyl peptide.

13. The method of claim 12 wherein the receptor expression priming agent is N-fMLP.

14. The method of claim 2 wherein the chemiluminigenic substrate is a cyclic hydrazide.

15. The method of claim 14 wherein the cyclic hydrazide is a 2,3-dihydro-1,4-phthalazinedione.

16. The method of claim 15 wherein the cyclic hydrazide is selected from a group consisting of luminol, isoluminol and 7-dimethylamino-naphthalene-1,2-dicarbonic acid hydrazide.

17. The method of claim 2 wherein the chemiluminigenic substrate is a dioxetane precursor capable of reacting with singlet-multiplicity molecular oxygen to produce a dioxetane or a dioxetanone.

18. A method of evaluating the systemic immune reserve of a patient, comprising:

a) contacting a first portion of a phagocyte-containing biological sample from the patient with an opsonified oxidative metabolism stimulating agent and a chemiluminigenic substrate;

b) contacting a second portion of the sample with a receptor expression priming agent, an opsonified oxidative metabolism stimulating agent and a chemiluminigenic substrate;

c) measuring the chemiluminescence responses of the first and second portions of the sample; and

d) comparing the chemiluminescence responses of the first and second portions of the sample as an indication of the systemic immune reserve of the patient.

19. The method of claim 18 wherein the patient is a human or animal patient and the biological sample comprises a diluted or undiluted biological fluid selected from the group consisting of whole blood, synovial fluid, amniotic fluid, cerebrospinal fluid, peritoneal fluid, pleural fluid, pericardial fluid, and fluids derived therefrom.

20. The method of claim 19 wherein the biological sample is diluted or undiluted whole blood.

21. The method of claim 18 wherein the opsonified oxidative metabolism stimulating agent is coated with one or more of the members of the group consisting of immunoglobulin and complement-derived opsonins.

22. The method of claim 18 wherein the opsonified oxidative metabolism stimulating agent is selected from the group consisting of opsonified attenuated bacteria, opsonified attenuated yeast and opsonified synthetic materials capable of fixing complement or eliciting specific antibody expression.

23. The method of claim 22 wherein the opsonified oxidative metabolism stimulating agent is opsonified zymosan.

24. The method of claim 18 wherein the receptor expression priming agent is selected from the group consisting of C5a, C5a_desArg, N-formyl-methionyl peptides, leukotrienes, platelet activating factor, and myeloid colony stimulating factors.

25. The method of claim 24 wherein the receptor expression priming agent is C5a or C5a_desArg.

26. The method of claim 24 wherein the receptor expression priming agent is an N-formyl-methionyl peptide.

27. The method of claim 26 wherein the receptor expression priming agent is N-fMLP.

28. The method of claim 18 wherein the chemiluminigenic substrate is a cyclic hydrazide.

29. The method of claim 28 wherein the cyclic hydrazide is a 2,3-dihydro-1,4phthalazinedione.

30. The method of claim 29 wherein the cyclic hydrazide is selected from a group consisting of luminol, isoluminol and 7-dimethylamino-naphthalene-1,2-dicarbonic acid hydrazide.

31. The method of claim 18 wherein the chemiluminigenic substrate is a dioxetane precursor capable of reacting with singlet-multiplicity molecular oxygen to produce a dioxetane or a dioxetanone.

32. A method of evaluating the humoral-immune modulated specific activity of phagocytes in a phagocyte-containing biological sample of a patient, comprising:

a) contacting the sample with a receptor expression priming agent, an opsonified oxidative metabolism stimulating agent and a chemiluminigenic substrate,

b) measuring the chemiluminescence response of the sample,

c) determining the approximate number of phagocytes in the sample;

d) determining the specific chemiluminescence response per phagocyte in the sample as a measure of phagocyte specific activity; and

d) comparing the phagocyte specific activity with those of a range of controls.

33. The method of claim 32 wherein the patient is a human or animal patient and the biological sample comprises a diluted or undiluted biological fluid selected from the group consisting of whole blood, synovial fluid, amniotic fluid, cerebrospinal fluid, peritoneal fluid, pleural fluid, pericardial fluid, and fluids derived therefrom.

34. The method of claim 33 wherein the biological sample is diluted or undiluted whole blood.

35. The method of claim 32 wherein the opsonified oxidative metabolism stimulating agent is coated with one or more of the members of the group consisting of immunoglobulin and complement-derived opsonins.

36. The method of claim 32 wherein the opsonified oxidative metabolism stimulating agent is selected from the group consisting of opsonified attenuated bacteria, opsonified attenuated yeast and opsonified synthetic materials capable of fixing complement or eliciting specific antibody expression.

37. The method of claim 36 wherein the opsonified oxidative metabolism stimulating agent is opsonified zymosan.

38. The method of claim 32 wherein the receptor expression priming agent is selected from the group consisting of C5a, C5a_desArg, N-formyl-methionyl peptides, leukotrienes, platelet activating factor, and myeloid colony stimulating factors.

39. The method of claim 38 wherein the receptor expression priming agent is C5a or C5a_desArg.

40. The method of claim 38 wherein the receptor expression priming agent is an N-formyl-methionyl peptide.

41. The method of claim 39 wherein the receptor expression priming agent is N-fMLP.

42. The method of claim 32 wherein the chemiluminigenic substrate is a cyclic hydrazide.

43. The method of claim 42 wherein the cyclic hydrazide is a 2,3-dihydro-1,4-phthalazinedione.

44. The method of claim 43 wherein the cyclic hydrazide is selected from a group consisting of luminol and 7-dimethylamino-naphthalene-1,2-dicarbonic acid hydrazide.

45. The method of claim 32 wherein the chemiluminigenic substrate is a dioxetane precursor capable of reacting with singlet-multiplicity molecular oxygen to produce a dioxetane or a dioxetanone.

46. A kit for use in the evaluation of the in vivo state of inflammation of a patient or the humoral-immune modulated specific activity of phagocytes comprising:

a) an opsonin receptor expression priming agent in an amount sufficient to obtain maximal expression of opsonin receptors on said phagocytes but not so high as to result in direct, opsonin receptor-independent oxidative activity;

b) an opsonified oxidative metabolism stimulating agent; and

c) a chemiluminigenic substrate.

47. The method of claim 46 wherein the opsonin receptor expression priming agent is selected from the group consisting of C5a, C5a_desArg, N-formyl-methionyl peptides, leukotrienes, platelet activating factor, and myeloid colony stimulating factors.

48. The kit of claim 47 wherein the receptor expression priming agent is C5a or C5a_desArg.

49. The kit of claim 47 wherein the receptor expression priming agent is an N-formyl-methionyl peptide.

50. The kit of claim 49 wherein the receptor expression priming agent is N-fMLP.

51. The kit of claim 46 wherein the opsonin receptor expression priming agent is coated on at least a portion of a light-transmissive container.

52. The kit of claim 46 wherein the opsonified oxidative metabolism stimulating agent is coated with one or more of the members of the group consisting of immunoglobulin and complement-derived opsonins.

53. The kit of claim 46 wherein the opsonified oxidative metabolism stimulating agent is selected from the group consisting of opsonified attenuated bacteria, opsonified attenuated yeast and opsonified synthetic materials capable of fixing complement or eliciting specific antibody expression.

54. The kit of claim 53 wherein the opsonified oxidative metabolism stimulating agent is opsonified zymosan.

55. The kit of claim 46 wherein the chemiluminigenic substrate is a cyclic hydrazide.

56. The kit of claim 55 wherein the cyclic hydrazide is a 2,3-dihydro-1,4-phthalazinedione.

57. The kit of claim 56 wherein the cyclic hydrazide is selected from a group consisting of luminol, isoluminol and 7-dimethylamino-naphthalene-1,2-dicarbonic acid hydrazide.

58. The kit of claim 46 wherein the chemiluminigenic substrate is a dioxetane precursor capable of reacting with singlet-multiplicity molecular oxygen to produce a dioxetane or a dioxetanone.

59. The kit of claim 51 having at least about 1 pmol of the opsonin receptor expression priming agent coated on the surface of said container.

60. The kit of claim 51 wherein the opsonin receptor expression priming agent is selected from the group consisting of C5a, C5a_desArg, N-formyl-methionyl peptides, leukotrienes, platelet activating factor, and myeloid colony stimulating factors.