DNA sequences encoding BMP-1 products

• US 5,108,922 A
• Filed: 07/31/1990
• Issued: 04/28/1992
• Est. Priority Date: 07/01/1986
• Status: Expired due to Fees

• Current Assignee: Genetics Institute LLC (Pfizer Inc.)
• Original Assignee: Genetics Institute LLC (Pfizer Inc.)
• Inventors: Wozney, John M., Rosen, Vicki, Wang, Elizabeth A.
• Primary Examiner(s): Stone, Jacqueline
• Assistant Examiner(s): Low, Christopher S. F.
• Application Number:
  US07/561,496
• Time in Patent Office: 637 Days
• Field of Search: 435/320, 435/252.33, 435/172.3, 435/91, 435/69.1, 435/70.3, 435/240.2, 435/320.1, 530/300, 530/350, 514/12, 536/27
• US Class Current: 435/365.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61L 24/043 Mixtures of macromolecular materials
• CPC Class Codes: A61L 27/227 Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
• CPC Class Codes: C07K 14/51 Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
• CPC Class Codes: C08L 89/00 Compositions of proteins; Compositions of derivatives thereof foodstuff preparations A23J3/00

Abstract

Purified BMP-1 proteins and processes for producing them are disclosed. They may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.

Claims

1. An isolated encoding a BMP-1 protein.

2. A host cell transformed with a DNA of claim 1.

3. An isolated DNA encoding a BMP-1 protein which is characterized by the ability to induce the formation of bone and/or cartilage said DNA comprising a coding sequence selected from the group consisting of:

(a) nucleotide #294 through nucleotide #404 of FIG. 1;

(b) nucleotide #3440 through nucleotide #3550 of FIG. 2;

(c) nucleotide #36 through nucleotide #2225 of FIG. 3; and

(d) naturally occurring allelic sequences and equivalent degenerative codon sequences of (a), (b), and (c).

4. A vector comprising a DNA of claim 3 in operative association with an expression control sequence therfor.

5. A host cell transformed with a vector of claim 4.

6. An isolated DNA encoding a BMP-1 protein said DNA comprising nucleotide #36 through nucleotide #2225 of FIG. 3.

7. A vector comprising the DNA of claim 6.

8. A host cell transformed with vector of claim 7.