Specimen transport device containing a specimen stabilizing composition

• US 5,108,927 A
• Filed: 11/09/1988
• Issued: 04/28/1992
• Est. Priority Date: 09/04/1985
• Status: Expired due to Fees

• Current Assignee: Gordon L. Dorn
• Original Assignee: Wadley Technologies, Inc.
• Inventors: Dorn, Gordon L.
• Primary Examiner(s): Lacey, David L.
• Assistant Examiner(s): Chan, William K. Y.
• Application Number:
  US07/269,490
• Time in Patent Office: 1,266 Days
• Field of Search: 435/4, 435/29, 435/34, 435/296, 435/286, 435/284, 435/243, 435/299, 128/760, 128/764, 128/765, 604/214, 604/220
• US Class Current: 435/307.1
• CPC Class Codes: C12M 33/04 by injection or suction, e.g. using pipettes, syringes, needles pipettes in general B01L3/02
• CPC Class Codes: C12N 1/04 Preserving or maintaining viable microorganisms immobilised microorganisms C12N11/00

Abstract

The improvement of specimen quality for microbial analysis is addressed by the present invention which discloses a chemical composition for use in a method and apparatus for transporting a specimen suspected to contain microorganisms of interest to a laboratory for analysis and improved methods of analysis.

A device and method for taking, storing, and preserving fluid samples is disclosed comprising a container and a composition designed to maintain the level of microorganisms present in a specimen during transportation of the specimen to a testing facility.

The method and apparatus can be utilized on all types of aqueous specimens and specimens which may be extracted in aqueous solution for analysis of microorganisms therein.

Claims

1. An apparatus for the collection and treatment of microorganisms in a specimen comprising:

(a) a container of generally elongated shape having a first end and a second end;

(b) a piston slidably mounted in said container;

(c) a shaft attached to said piston and extending through said first end, said shaft includes a narrow portion causing said shaft to break at said narrow portion when said shaft is bent;

(d) a detachable cap removably attached to said second end

said cap, said container and said piston forming a sealed chamber, in which the pressure in the chamber may be decreased by moving said piston toward said first end;

said cap being puncturable for insertion of said specimen, and;

(e) a water-soluble specimen transport system deposited in said container, said specimen transport system comprising a water-soluble additive at a concentration effective to prevent replication of microorganisms present in said specimen, when said specimen is mixed with said water additive therein to form a solution, and reducing the cidal activity toward said microorganisms of antimicrobial factors present in said specimen so that at least some microorganisms will be capable of replication upon dilution of said solution on medium capable of supporting replication of said microorganisms.

2. The apparatus according to claim 1, wherein said apparatus further comprises a means to lock said piston to prevent movement of said piston within said container, said means to lock comprises the narrow portion of said shift causing the shaft to break when said shaft is bent.

3. The apparatus of claim 1, further comprising:

(f) a substance effective for preserving the viability of microorganisms of interest in said specimen in said container.

4. The apparatus of claim 3, wherein said substance for preserving the viability of said microorganisms of interest comprises a growth base effective for supporting the general nutritional needs of the microorganisms of interest.

5. The apparatus according to claim 3, wherein said substance for preserving the viability of the microorganisms of interest in the specimen comprises starch.

6. The apparatus according to claim 3, wherein said substance for preserving the viability of the microorganisms of interest in th specimen comprises agar.

7. The apparatus according to claim 3, wherein said substance for preserving the viability of the microorganisms of interest in the specimen comprises hemoglobin.

8. An apparatus for the collection and treatment of microorganisms in a specimen comprising:

(a) a container of generally elongated shape having a first end and a second end;

(b) a piston slidably mounted in said container;

(c) a shaft attached to said piston and extending through said first end, said shaft being detachably connected to said piston by complementary thread means;

(d) a detachable cap removably attached to said second end

said cap, said container and said piston forming a sealed chamber, in which the pressure in the chamber may be decreased by moving said piston toward said first end;

said cap being puncturable for insertion of said specimen, and;

(e) a water-soluble specimen transport system deposited in said container, said specimen transport system comprising a water-soluble additive at a concentration effective to prevent replication of microorganisms present in said specimen, when said specimen is mixed with said water additive therein to form a solution, and reducing the cidal activity toward said microorganisms of antimicrobial factors present in said specimen so that at least some microorganisms will be capable of replication upon dilution of said solution on medium capable of supporting replication of said microorganisms.

9. The apparatus of claim 8 wherein said apparatus further comprises a means to lock said piston to prevent movement of said piston within said container, said means to lock comprises the complementary thread means allowing detachment of said shaft from said piston.

10. The apparatus of claim 8, further comprising:

(f) a substance effective for preserving the viability of microorganisms of interest in said specimen in said container.

11. The apparatus of claim 10, wherein said substance for preserving the viability of said microorganisms of interest comprises a growth base effective for supporting the general nutritional needs of the microorganisms of interest.

12. The apparatus according to claim 10, wherein said substance for preserving the viability of the microorganisms of interest in the specimen comprises starch.

13. The apparatus according to claim 10, wherein said substance for preserving the viability of the microorganisms of interest in the specimen comprises agar.

14. The apparatus according to claim 10, wherein said substance for preserving the viability of the microorganisms of interest in the specimen comprises hemoglobin.