Methods of detecting glycated proteins
First Claim
1. A method of detecting glycated protein in a body fluid suspected of containing said glycated protein, comprising the steps of:
- a) contacting a test sample of said body fluid with an excess of a binder composition under conditions which allow binding of glycated protein in said test sample to a portion of said binder composition thereby leaving a portion of said binder composition unbound with glycated protein;
b) exposing the unbound portion of said binder composition from step a to an excess of glycated compound affixed to a solid support under conditions which allow reaction of said unbound portion of said binder composition to a portion of said glycated compound affixed to a solid support thereby leaving a portion of said glycated compound affixed to a solid support unreacted with said unbound portion of said binder composition;
c) reacting the unreacted portion of said glycated compound affixed to a solid support from step b with a detecting agent; and
d) detecting said detecting agent.
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Accused Products
Abstract
The invention discloses novel methods of detecting glycated proteins in test samples of blood and methods of detecting chronic hyperglycemia. The test sample is contacted with a binder composition under conditions which allow binding of glycated protein in the test sample to at least a portion of the binder composition. The test sample is then exposed to a glycated compound affixed to a solid support under conditions which allow raction of unbound binder composition from the previous step to at least a portion of the glycated compound on the solid support. The glycated compound affixed to a solid support from the previous step which did not react with the unbound binder composition is then reacted with a detecting agent and the detecting agent is detected.
114 Citations
33 Claims
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1. A method of detecting glycated protein in a body fluid suspected of containing said glycated protein, comprising the steps of:
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a) contacting a test sample of said body fluid with an excess of a binder composition under conditions which allow binding of glycated protein in said test sample to a portion of said binder composition thereby leaving a portion of said binder composition unbound with glycated protein; b) exposing the unbound portion of said binder composition from step a to an excess of glycated compound affixed to a solid support under conditions which allow reaction of said unbound portion of said binder composition to a portion of said glycated compound affixed to a solid support thereby leaving a portion of said glycated compound affixed to a solid support unreacted with said unbound portion of said binder composition; c) reacting the unreacted portion of said glycated compound affixed to a solid support from step b with a detecting agent; and d) detecting said detecting agent. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. A method of detecting chronic hyperglycemia in mammals, comprising
a) contacting a test sample of a body fluid from a mammal suspected of containing glycated protein with an excess of a binder composition under conditions which allow binding of glycated protein in said test sample to a portion of said binder composition thereby leaving a portion of said binder composition unbound with glycated protein; -
b) exposing the unbound portion of said binder composition from step a to an excess of glycated compound affixed to a solid support under conditions which allow reaction of said unbound portion of said binder composition to a portion of said glycated compound affixed to a solid support thereby leaving a portion of said glycated compound affixed to a solid support unreacted with said unbound portion of said binder composition; c) reacting the unreacted portion of said glycated compound affixed to a solid support from step b with a detecting agent; and d) detecting said detecting agent whereby the presence of detecting agent indicates the presence of glycated protein in said test sample and the presence in said mammal of chronic hyperglycemia. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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Specification