Preparation of synthetic enzymes and synthetic antibodies and use of the thus prepared enzymes and antibodies

• US 5,110,833 A
• Filed: 03/29/1990
• Issued: 05/05/1992
• Est. Priority Date: 01/16/1989
• Status: Expired due to Term

• Current Assignee: Mosbach, Klaus
• Original Assignee: Mosbach, Klaus
• Inventors: Mosbach, Klaus
• Primary Examiner(s): Foelak, Morton
• Application Number:
  US07/500,892
• Time in Patent Office: 768 Days
• Field of Search: 521/142, 521/149, 521/61, 521/64, 521/150, 525/54.1, 435/183, 530/387
• US Class Current: 521/50
• CPC Class Codes: B01J 20/268 Polymers created by use of a template, e.g. molecularly imprinted polymers
• CPC Class Codes: C12N 9/00 Enzymes; Proenzymes; Compositions thereof preparations containing enzymes for cleaning teeth A61K8/66, A61Q11/00; medicinal preparations containing enzymes or proenzymes A61K38/43; enzyme containing detergent compositions C11D; enzymes with nucleic acid structure, e.g. ribozymes, C12N15/113; Processes for preparing, activating, inhibiting, separating or purifying enzymes preparation of malt C12C1/00
• CPC Class Codes: C12P 13/02 Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes peptides C12P21/00 or C07K
• CPC Class Codes: C12P 7/22 aromatic
• CPC Class Codes: G01N 2600/00 Assays involving molecular imprinted polymers/polymers created around a molecular template

Abstract

A method of preparing synthetic enzymes and synthetic antibodies in which method use is made of the so called molecular imprinting method, is described. As imprinting molecules use is made of transition state analogues, substrate-like compounds and antigen-like molecules, respectively. There is also described the use of the so prepared enzymes and antibodies.

Claims

1. A method of producing synthetic enzymes or synthetic antibodies, comprising the orientation of monomers around a print molecule, addition of crosslinkers, polymerizing to a polymer and subsequent removal of the print molecule, thereby creating a cavity in the polymer corresponding to said print molecule.

3. A method according to claim 1 or 2 for producing enzymes, whereby as print molecule use is made of a transition state analogue.

4. A method according to claim 1 or 2 for producing enzymes, whereby as print molecule use is made of a substrate-like compound.

5. A method according to claim 1 for producing synthetic antibodies, whereby as print molecule use is made of an antigen-like compound.

2. A method of producing synthetic enzymes, comprising polymerisation of monomers to a polymer around a print molecule and thereafter removing the print molecule, thereby creating a cavity in the polymer corresponding to said print molecule.